Li Qingyun, Karnowski Karol, Noble Peter B, Cairncross Alvenia, James Alan, Villiger Martin, Sampson David D
Optical + Biomedical Engineering Laboratory, Department of Electrical, Electronic and Computer Engineering, The University of Western Australia, Perth, WA 6009, Australia.
School of Human Sciences, The University of Western Australia, Perth, WA 6009, Australia.
Biomed Opt Express. 2018 Oct 15;9(11):5437-5455. doi: 10.1364/BOE.9.005437. eCollection 2018 Nov 1.
It is challenging to recover local optic axis orientation from samples probed with fiber-based polarization-sensitive optical coherence tomography (PS-OCT). In addition to the effect of preceding tissue layers, the transmission through fiber and system elements, and imperfect system alignment, need to be compensated. Here, we present a method to retrieve the required correction factors from measurements with depth-multiplexed PS-OCT, which accurately measures the full Jones matrix. The correction considers both retardation and diattenuation and is applied in the wavenumber domain, preserving the axial resolution of the system. The robustness of the method is validated by measuring a birefringence phantom with a misaligned system. Imaging lamb trachea and human bronchus demonstrates the utility of reconstructing the local optic axis orientation to assess smooth muscle, which is expected to be useful in the assessment of airway smooth muscle thickness in asthma, amongst other fiber-based applications.
从基于光纤的偏振敏感光学相干断层扫描（PS-OCT）探测的样本中恢复局部光轴方向具有挑战性。除了前面组织层的影响外，还需要补偿通过光纤和系统元件的传输以及不完美的系统对准。在此，我们提出一种方法，可从深度复用PS-OCT的测量中检索所需的校正因子，该方法可精确测量完整的琼斯矩阵。该校正同时考虑了相位延迟和二向色性，并在波数域中应用，从而保留了系统的轴向分辨率。通过使用未对准系统测量双折射模型验证了该方法的稳健性。对羊气管和人支气管进行成像，证明了重建局部光轴方向以评估平滑肌的实用性，这有望在评估哮喘中的气道平滑肌厚度以及其他基于光纤的应用中发挥作用。
