Australian Regenerative Medicine Institute, Monash University, Victoria, Australia.
Dev Dyn. 2011 Feb;240(2):422-31. doi: 10.1002/dvdy.22537. Epub 2011 Jan 11.
Laminins are essential components of all basement membranes and are fundamental to tissue development and homeostasis. Humans possess at least 16 different heterotrimeric laminin complexes formed through different combinations of alpha, beta, and gamma chains. Individual chains appear to exhibit unique expression patterns, leading to the notion that overlap between expression domains governs the constitution of complexes found within particular tissues. However, the spatial and temporal expression of laminin genes has not been comprehensively analyzed in any vertebrate model to date. Here, we describe the tissue-specific expression patterns of all laminin genes in the zebrafish, throughout embryonic development and into the "post-juvenile" period, which is representative of the adult body form. In addition, we present phylogenetic and microsynteny analyses, which demonstrate that the majority of our zebrafish sequences are orthologous to human laminin genes. Together, these data represent a fundamental resource for the study of vertebrate laminins.
层粘连蛋白是所有基底膜的基本组成部分，对组织发育和内稳态至关重要。人类至少拥有 16 种不同的异三聚体层粘连蛋白复合物，这些复合物是通过不同的α、β和γ链组合形成的。各个链似乎表现出独特的表达模式，这导致了表达域之间的重叠控制着在特定组织中发现的复合物的组成的概念。然而，到目前为止，在任何脊椎动物模型中，层粘连蛋白基因的时空表达都没有得到全面分析。在这里，我们描述了斑马鱼中所有层粘连蛋白基因在胚胎发育过程中的组织特异性表达模式，并进入了“幼体后”阶段，这一阶段代表了成年体型。此外，我们还进行了系统发育和微同线性分析，证明我们的大多数斑马鱼序列与人类层粘连蛋白基因是同源的。这些数据共同构成了研究脊椎动物层粘连蛋白的基础资源。
