Wnt5a 调控发育中鼠牙的生长、形态发生和成牙本质细胞分化。
Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth.
机构信息
Department of Periodontology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, Fujian, China.
出版信息
Dev Dyn. 2011 Feb;240(2):432-40. doi: 10.1002/dvdy.22550.
Abstract
Wnt/β-catenin signaling is essential for tooth development beyond the bud stage, but little is known about the role of non-canonical Wnt signaling in odontogenesis. Here we compared the expression of Wnt5a, a representative of noncanonical Wnts, with that of Ror2, the Wnt5a receptor for non-canonical signaling, in the developing tooth, and analyzed tooth phenotype in Wnt5a mutants. Wnt5a-deficient mice exhibit retarded tooth development beginning from E16.5, leading to the formation of smaller and abnormally patterned teeth with a delayed odontoblast differentiation at birth. These defects are associated with upregulated Axin2 and Shh expression in the dental epithelium and reduced levels of cell proliferation in the dental epithelium and mesenchyme. Retarded tooth development and defective odontoblast differentiation were also observed in Ror2 mutant mice. Our results suggest that Wnt5a regulates growth, patterning, and odontoblast differentiation during odontogenesis, at least partially by modulating Wnt/β-catenin canonical signaling.
摘要
Wnt/β-catenin 信号通路对于芽期后的牙齿发育至关重要，但关于非经典 Wnt 信号通路在牙齿发生中的作用知之甚少。在这里，我们比较了非经典 Wnt 的代表 Wnt5a 与非经典信号的 Wnt5a 受体 Ror2 在发育中的牙齿中的表达，并分析了 Wnt5a 突变体的牙齿表型。Wnt5a 缺陷型小鼠从 E16.5 开始表现出牙齿发育迟缓，导致出生时形成更小且异常模式的牙齿，牙本质细胞分化延迟。这些缺陷与牙上皮中 Axin2 和 Shh 表达上调以及牙上皮和间充质中细胞增殖减少有关。Ror2 突变型小鼠也观察到牙齿发育迟缓及成牙本质细胞分化缺陷。我们的结果表明，Wnt5a 通过调节 Wnt/β-catenin 经典信号通路，在牙齿发生过程中调节牙齿的生长、形态和牙本质细胞分化。
相似文献
1
Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth.Wnt5a 调控发育中鼠牙的生长、形态发生和成牙本质细胞分化。
Dev Dyn. 2011 Feb;240(2):432-40. doi: 10.1002/dvdy.22550.
2
Wntless regulates dentin apposition and root elongation in the mandibular molar.无翅型MMTV整合位点家族蛋白调节下颌磨牙的牙本质附着和牙根伸长。
J Dent Res. 2015 Mar;94(3):439-45. doi: 10.1177/0022034514567198. Epub 2015 Jan 16.
3
The Wnt5a/Ror2 noncanonical signaling pathway inhibits canonical Wnt signaling in K562 cells.Wnt5a/Ror2 非经典信号通路抑制 K562 细胞中的经典 Wnt 信号通路。
Int J Mol Med. 2011 Jan;27(1):63-9. doi: 10.3892/ijmm.2010.560. Epub 2010 Nov 10.
4
Wnt/beta-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development.Wnt/β-连环蛋白信号通路在牙齿早期发育过程中牙源性间充质的激活中起重要作用。
Dev Biol. 2009 Oct 1;334(1):174-85. doi: 10.1016/j.ydbio.2009.07.015. Epub 2009 Jul 22.
5
DPP an extracellular matrix molecule induces Wnt5a mediated signaling to promote the differentiation of adult stem cells into odontogenic lineage.DPP 是一种细胞外基质分子，它诱导 Wnt5a 介导的信号通路，促进成体干细胞向牙源性谱系分化。
Sci Rep. 2024 Oct 31;14(1):26187. doi: 10.1038/s41598-024-76069-7.
6
Enhanced Wnt/β-catenin signalling during tooth morphogenesis impedes cell differentiation and leads to alterations in the structure and mineralisation of the adult tooth.牙发生过程中 Wnt/β-连环蛋白信号的增强会阻碍细胞分化，并导致成年牙齿的结构和矿化改变。
Biol Cell. 2012 Oct;104(10):603-17. doi: 10.1111/boc.201100075. Epub 2012 Aug 23.
7
The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway.梅克尔-格鲁伯综合征蛋白TMEM67通过非经典Wnt信号通路控制小鼠的基体定位和上皮分支形态发生。
Dis Model Mech. 2015 Jun;8(6):527-41. doi: 10.1242/dmm.019083. Epub 2015 Apr 7.
8
SMAD4-mediated WNT signaling controls the fate of cranial neural crest cells during tooth morphogenesis.SMAD4 介导的 WNT 信号在牙齿形态发生过程中控制颅神经嵴细胞的命运。
Development. 2011 May;138(10):1977-89. doi: 10.1242/dev.061341. Epub 2011 Apr 13.
9
Wnt5a mediated canonical Wnt signaling pathway activation in orthodontic tooth movement: possible role in the tension force-induced bone formation.Wnt5a介导正畸牙齿移动中经典Wnt信号通路的激活：在张力诱导的骨形成中的可能作用。
J Mol Histol. 2016 Oct;47(5):455-66. doi: 10.1007/s10735-016-9687-y. Epub 2016 Jul 25.
10
Enamel knots as signaling centers linking tooth morphogenesis and odontoblast differentiation.釉结作为连接牙齿形态发生和成牙本质细胞分化的信号中心。
Adv Dent Res. 2001 Aug;15:14-8. doi: 10.1177/08959374010150010401.
引用本文的文献
1
Wnt5a gain- and loss-of-function present distinctly in craniofacial bone.Wnt5a功能获得和功能缺失在颅面骨中表现明显不同。
bioRxiv. 2025 Jul 22:2025.07.21.665966. doi: 10.1101/2025.07.21.665966.
2
Developmental basis of natural tooth shape variation in cichlid fishes.丽鱼科鱼类天然牙齿形状变异的发育基础。
Naturwissenschaften. 2025 Jan 27;112(1):12. doi: 10.1007/s00114-025-01964-6.
3
The sensory nerve regulates stem cell homeostasis through Wnt5a signaling.感觉神经通过Wnt5a信号传导调节干细胞稳态。
iScience. 2024 Sep 26;27(10):111035. doi: 10.1016/j.isci.2024.111035. eCollection 2024 Oct 18.
4
DPP an extracellular matrix molecule induces Wnt5a mediated signaling to promote the differentiation of adult stem cells into odontogenic lineage.DPP 是一种细胞外基质分子，它诱导 Wnt5a 介导的信号通路，促进成体干细胞向牙源性谱系分化。
Sci Rep. 2024 Oct 31;14(1):26187. doi: 10.1038/s41598-024-76069-7.
5
Integrated MicroRNA-mRNA Analyses of the Osteogenic Differentiation of Human Dental Pulp Stem Cells by a Helioxanthin Derivative.一种海胆黄质衍生物对人牙髓干细胞成骨分化的MicroRNA-mRNA综合分析
Curr Issues Mol Biol. 2024 Sep 28;46(10):10960-10968. doi: 10.3390/cimb46100651.
6
The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes.牙间充质干细胞的成牙本质细胞分化：分子调控机制及相关遗传综合征
Front Cell Dev Biol. 2023 Sep 25;11:1174579. doi: 10.3389/fcell.2023.1174579. eCollection 2023.
7
Dental abnormalities in rare genetic bone diseases: Literature review.罕见遗传性骨病的牙齿异常：文献综述。
Clin Anat. 2024 Apr;37(3):304-320. doi: 10.1002/ca.24117. Epub 2023 Sep 22.
8
Effect of Wnt10a/β-catenin signaling pathway on promoting the repair of different types of dentin-pulp injury.Wnt10a/β-catenin 信号通路对促进不同类型牙本质-牙髓损伤修复的作用。
In Vitro Cell Dev Biol Anim. 2023 Aug;59(7):486-504. doi: 10.1007/s11626-023-00785-z. Epub 2023 Sep 12.
9
ROR2-Related Skeletal Dysplasia Reveals Disrupted Chondrocyte Polarity through Modulation of BMP/TGF-β Signaling.ROR2 相关骨骼发育不良通过调节 BMP/TGF-β 信号通路破坏软骨细胞极性。
Aging Dis. 2024 Feb 1;15(1):282-294. doi: 10.14336/AD.2023.0531.
10
Effects of and Double Mutations on Tooth Development.双突变对牙齿发育的影响。
Genes (Basel). 2023 Jan 28;14(2):340. doi: 10.3390/genes14020340.
本文引用的文献
1
Inhibition of Wnt signaling by Wise (Sostdc1) and negative feedback from Shh controls tooth number and patterning.Wnt 信号的抑制由 Wise（Sostdc1）和 Shh 的负反馈控制牙齿数量和模式。
Development. 2010 Oct;137(19):3221-31. doi: 10.1242/dev.054668. Epub 2010 Aug 19.
2
Wnt5a promotes differentiation of human dental papilla cells.Wnt5a 促进人牙髓细胞的分化。
Int Endod J. 2010 May;43(5):404-12. doi: 10.1111/j.1365-2591.2010.01693.x.
3
Wnt/beta-catenin signaling in oral tissue development and disease.Wnt/β-连环蛋白信号通路在口腔组织发育和疾病中的作用。
J Dent Res. 2010 Apr;89(4):318-30. doi: 10.1177/0022034510363373. Epub 2010 Mar 3.
4
Expression of Wnt5a in tooth germs and the related signal transduction analysis.Wnt5a 在牙胚中的表达及相关信号转导分析。
Arch Oral Biol. 2010 Feb;55(2):108-14. doi: 10.1016/j.archoralbio.2009.12.002.
5
Towards an integrated view of Wnt signaling in development.迈向发育中Wnt信号通路的整合观点。
Development. 2009 Oct;136(19):3205-14. doi: 10.1242/dev.033910.
6
Expression of Axin2 indicates a role for canonical Wnt signaling in development of the crown and root during pre- and postnatal tooth development.Axin2 的表达表明经典 Wnt 信号在牙齿发育的出生前和出生后阶段的牙冠和牙根发育中发挥作用。
Dev Dyn. 2010 Jan;239(1):160-7. doi: 10.1002/dvdy.22047.
7
Wnt/beta-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development.Wnt/β-连环蛋白信号通路在牙齿早期发育过程中牙源性间充质的激活中起重要作用。
Dev Biol. 2009 Oct 1;334(1):174-85. doi: 10.1016/j.ydbio.2009.07.015. Epub 2009 Jul 22.
8
Wnt5a regulates directional cell migration and cell proliferation via Ror2-mediated noncanonical pathway in mammalian palate development.在哺乳动物腭部发育过程中，Wnt5a通过Ror2介导的非经典途径调节细胞的定向迁移和增殖。
Development. 2008 Dec;135(23):3871-9. doi: 10.1242/dev.025767. Epub 2008 Oct 23.
9
Axin2 expression identifies progenitor cells in the murine prostate.Axin2表达可识别小鼠前列腺中的祖细胞。
Prostate. 2008 Sep 1;68(12):1263-72. doi: 10.1002/pros.20770.
10
Regulation of epithelial branching morphogenesis and cancer cell growth of the prostate by Wnt signaling.Wnt信号通路对前列腺上皮分支形态发生和癌细胞生长的调控
PLoS One. 2008 May 14;3(5):e2186. doi: 10.1371/journal.pone.0002186.
Zotero 插件