Mazyad Said A M, Shoukry Nahla M, El-Alfy Nadia M
Research and Training Center on Vector of Diseases, Ain Shams University, Cairo, Egypt.
J Egypt Soc Parasitol. 2010 Aug;40(2):499-514.
Ticks and blood samples were collected every month from March 2009 through April 2010 from different sites in Sinai to detect babesial parasites using PCR assay based on nuclear small subunit rRNA gene. Ticks were found to contain babesial DNA. Sequence determination and analysis of amplified portions of nss-rDNA revealed their identity with B. bovis and a high degree of homology with B. bigemina and B. divergens. The results represent the first genetic evidence of different species of Babesia and identified the role of Ixodes ricinus as a vector of zoonotic B. microti infection. Rodent isolate (HK) and American isolate (GI) were studied in transmission experiments. The present study used in vitro culture of zoonotic Babesia sp. EU1 from blood samples of rodent in Sinai. This study provides an evidence of transovarian and transstadial transmissions of the parasite within I. ricinus, which emphasizes that this tick could be a vector and reservoir of EU1
从2009年3月至2010年4月，每月从西奈半岛的不同地点采集蜱虫和血液样本，采用基于核小亚基rRNA基因的PCR检测法检测巴贝斯虫寄生虫。发现蜱虫含有巴贝斯虫DNA。对nss-rDNA扩增片段进行序列测定和分析，结果显示它们与牛巴贝斯虫相同，与双芽巴贝斯虫和分歧巴贝斯虫高度同源。这些结果是不同巴贝斯虫物种的首个遗传学证据，并确定蓖麻硬蜱作为人兽共患微小巴贝斯虫感染传播媒介的作用。在传播实验中对啮齿动物分离株（HK）和美国分离株（GI）进行了研究。本研究使用了从西奈半岛啮齿动物血液样本中分离出的人兽共患巴贝斯虫属EU1进行体外培养。该研究提供了寄生虫在蓖麻硬蜱体内经卵传递和经变态传递的证据，这强调了这种蜱可能是EU1的传播媒介和储存宿主。
