Nucleus for Research in Environmental Sciences, Center for Sciences and Technology, Catholic University of Pernambuco, Boa Vista, Recife-PE, Brazil.
Colloids Surf B Biointerfaces. 2011 May 1;84(1):1-5. doi: 10.1016/j.colsurfb.2010.10.045. Epub 2010 Nov 5.
In the last years, researches developed with biosurfactants for application in the medical area have been revealing the promising biological activities of these biomolecules. In this work the antimicrobial and anti-adhesive properties of a biosurfactant Rufisan isolated from the yeast Candida lipolytica UCP 0988, growth in a medium supplemented with ground nut refinery residue was determined against several microorganisms. The biosurfactant was able to reduce the water surface tension from 70 to 25.3 mN/m and showed a critical micelle concentration (CMC) of 0.03%. The biosurfactant was isolated after 72 h of fermentation and was tested in concentrations varying from 0.75 to 12 mg/l. The highest antimicrobial activities were observed against Streptococcus agalactiae, Streptococcus mutans, Streptococcus mutans NS, Streptococcus mutans HG, Streptococcus sanguis 12, Streptococcus oralis J22 at a concentration superior to the biosurfactant critical micelle concentration. Moreover, the biosurfactant showed anti-adhesive activity against most of the microorganisms tested. As far as we know, this is the first compilation of data on antimicrobial and anti-adhesive activities of a biosurfactant obtained from a Candida strain against such a broad group of microorganisms. The results obtained in this work showed that the biosurfactant from C. lipolytica is a potential antimicrobial and/or anti-adhesive agent for several biomedical applications.
在过去的几年中，研究人员开发了用于医学领域的生物表面活性剂，这些生物分子的有前途的生物活性正在被揭示。在这项工作中，从酵母 Candida lipolytica UCP 0988 中分离出的生物表面活性剂 Rufisan 的抗菌和抗粘附特性，在补充了花生精炼厂残渣的培养基中生长，针对几种微生物进行了测定。生物表面活性剂能够将水的表面张力从 70 降低到 25.3 mN/m，并且表现出临界胶束浓度 (CMC) 为 0.03%。生物表面活性剂在发酵 72 小时后被分离出来，并在 0.75 至 12 mg/l 的浓度下进行测试。在高于生物表面活性剂临界胶束浓度的浓度下，观察到对酿脓链球菌、变形链球菌、变形链球菌 NS、变形链球菌 HG、血链球菌 12、口腔链球菌 J22 的最高抗菌活性。此外，生物表面活性剂对大多数测试的微生物表现出抗粘附活性。据我们所知，这是第一个关于从 Candida 菌株获得的生物表面活性剂对如此广泛的微生物群的抗菌和/或抗粘附活性的数据汇编。这项工作的结果表明，C. lipolytica 的生物表面活性剂是几种生物医学应用的潜在抗菌和/或抗粘附剂。
