Rodrigues Lígia R, Teixeira José A, van der Mei Henny C, Oliveira Rosário
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Colloids Surf B Biointerfaces. 2006 Nov 1;53(1):105-12. doi: 10.1016/j.colsurfb.2006.08.009. Epub 2006 Aug 22.
Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved was 20 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, this glycolipid rich fraction was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered.
对嗜热链球菌A产生的粗生物表面活性剂中的表面活性成分进行了分离和表征研究。通过疏水相互作用色谱法对粗生物表面活性剂进行分馏，得到了富含糖脂的馏分。测定了分子组成（通过傅里叶变换红外光谱）和元素组成（通过X射线光电子能谱）。所达到的临界胶束浓度为20 g/l，表面张力值为36 mJ/m²。此外，发现这种富含糖脂的馏分对从植入的语音假体中分离出的几种细菌和酵母菌株具有抗粘附和抗菌作用。由于每个纯化步骤都会增加成本并减少回收的生物表面活性剂数量，因此应仔细分析进一步的纯化步骤。
