Department of Chemistry, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal.
J Colloid Interface Sci. 2011 Mar 15;355(2):512-7. doi: 10.1016/j.jcis.2010.12.071. Epub 2010 Dec 25.
Crosslinked κ-carrageenan hydrogel nanoparticles (nanogels) with an average size smaller than 100 nm were prepared using reverse microemulsions combined with thermally induced gelation. The size of the nanogels varied with biopolymer concentration at a constant water/surfactant concentration ratio. The nanogels were found to be thermo-sensitive in a temperature range acceptable for living cells (37-45°C) undergoing reversible volume transitions in response to thermal stimuli. This opens the possibility to explore the application of these nanogels in smart therapeutics such as thermo-sensitive drug carriers. As such, the sustained release of methylene blue from the nanogels was evaluated in in vitro conditions as proof of concept experiments and the release rate was found to be controlled with temperature.
采用反相乳液法结合热致凝胶法制备了平均粒径小于 100nm 的交联 κ-卡拉胶水凝胶纳米颗粒（纳米凝胶）。纳米凝胶的粒径随生物聚合物浓度的变化而变化，在生物细胞可接受的温度范围内（37-45°C），在恒定的水/表面活性剂浓度比下。纳米凝胶具有温度响应性，可发生可逆体积相变。这为探索纳米凝胶在智能治疗领域的应用提供了可能，如热敏药物载体。因此，在体外条件下评估了纳米凝胶中亚甲蓝的持续释放，作为概念验证实验，发现释放速率可以通过温度来控制。
