追踪成人干细胞。
Tracking adult stem cells.
机构信息
Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.
出版信息
EMBO Rep. 2011 Feb;12(2):113-22. doi: 10.1038/embor.2010.216. Epub 2011 Jan 21.
Abstract
The maintenance of stem-cell-driven tissue homeostasis requires a balance between the generation and loss of cell mass. Adult stem cells have a close relationship with the surrounding tissue--known as their niche--and thus, stem-cell studies should preferably be performed in a physiological context, rather than outside their natural environment. The mouse is an attractive model in which to study adult mammalian stem cells, as numerous experimental systems and genetic tools are available. In this review, we describe strategies commonly used to identify and functionally characterize adult stem cells in mice and discuss their potential, limitations and interpretations, as well as how they have informed our understanding of adult stem-cell biology. An accurate interpretation of physiologically relevant stem-cell assays is crucial to identify adult stem cells and elucidate how they self-renew and give rise to differentiated progeny.
摘要
维持干细胞驱动的组织稳态需要在细胞质量的产生和损失之间取得平衡。成体干细胞与周围组织（称为其生态位）密切相关，因此，最好在生理环境中进行干细胞研究，而不是在其自然环境之外进行。由于存在许多实验系统和遗传工具，因此小鼠是研究成年哺乳动物干细胞的一种有吸引力的模型。在这篇综述中，我们描述了常用于鉴定和功能表征小鼠中成体干细胞的策略，并讨论了它们的潜力、局限性和解释，以及它们如何帮助我们理解成体干细胞生物学。准确解释与生理相关的干细胞测定对于鉴定成体干细胞并阐明它们如何自我更新以及产生分化后代至关重要。
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本文引用的文献
1
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Nat Genet. 2011 Jan;43(1):34-41. doi: 10.1038/ng.722. Epub 2010 Nov 28.
2
Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts.潘氏细胞构成了肠道隐窝中 Lgr5 干细胞的龛位。
Nature. 2011 Jan 20;469(7330):415-8. doi: 10.1038/nature09637. Epub 2010 Nov 28.
3
Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor.刺猬信号通路调控不断生长的小鼠切牙中成釉器祖细胞的生成。
Development. 2010 Nov;137(22):3753-61. doi: 10.1242/dev.056358.
4
Stability of the regulatory T cell lineage in vivo.体内调节性 T 细胞谱系的稳定性。
Science. 2010 Sep 24;329(5999):1667-71. doi: 10.1126/science.1191996.
5
Intestinal stem cell replacement follows a pattern of neutral drift.肠干细胞的更替遵循中性漂移模式。
Science. 2010 Nov 5;330(6005):822-5. doi: 10.1126/science.1196236. Epub 2010 Sep 23.
6
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.肠隐窝稳态源自对称分裂的 Lgr5 干细胞之间的中性竞争。
Cell. 2010 Oct 1;143(1):134-44. doi: 10.1016/j.cell.2010.09.016.
7
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