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南极利文斯顿岛嗜冷酵母 Sporobolomyces salmonicolor AL₁ 合成的胞外多糖的生产及化学特性研究。

Production and chemical characterization of an exopolysaccharide synthesized by psychrophilic yeast strain Sporobolomyces salmonicolor AL₁ isolated from Livingston Island, Antarctica.

机构信息

Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, 80078 Pozzuoli, Italy.

出版信息

Folia Microbiol (Praha). 2010 Nov;55(6):576-81. doi: 10.1007/s12223-010-0092-8. Epub 2011 Jan 21.

Abstract

The exopolysaccharide (EPS) production by psychrophilic Antarctic yeast Sporobolomyces salmonicolor AL₁ reached the maximum yield in medium containing sucrose (50 g/L) and diammonium sulfate (2.5 g/L) after a 5-d fermentation (5.64 g/L) at 22 °C, the dynamic viscosity of the culture broth reaching (after 5 d) 15.4 mPa s. EPS showed a mannan-like structure and high molar mass, and did not affect cellular viability and proliferation of murine macrophages. It exhibited also a protective effect against the toxic activity of Avarol.

摘要

嗜冷南极酵母 Sporobolomyces salmonicolor AL₁ 的胞外多糖 (EPS) 生产在 22°C 下发酵 5 天后(5.64 g/L),在含有蔗糖(50 g/L)和硫酸二铵(2.5 g/L)的培养基中达到最大产量(5.64 g/L),培养液的动态黏度达到(5 天后)15.4 mPa·s。EPS 呈现甘露聚糖样结构和高摩尔质量,且不影响细胞活力和小鼠巨噬细胞的增殖。它还表现出对 Avarol 毒性活性的保护作用。

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