Zhang Zuo-Fa, Song Ting-Ting, Chen Jian-Fei, Lv Guo-Ying
Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, China.
Front Nutr. 2023 Jan 13;9:1095556. doi: 10.3389/fnut.2022.1095556. eCollection 2022.
In this study, an effective method was developed to extract the polysaccharide from (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)--Gal-(1→ and →2,6)--Man-(1→ and →2)--Fuc-(1→ and was branched of t--Man-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the , and genes and upregulated the and genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.
在本研究中，开发了一种通过破坏细胞壁从[具体名称未给出，推测为某种物质，暂用PFSV表示]中提取多糖的有效方法。采用Box-Behnken设计确定最佳工艺条件如下：加工温度（80°C）、加工时间（0.81小时）和盐酸用量（1.5毫升）。在此条件下，PFSV的产率达到5.94±0.16%。纯化后的多糖（PFSV-2）被发现是一种杂多糖，平均分子量为20.377 kDa。PFSV-2的主链由→6)--Gal-(1→、→2,6)--Man-(1→和→2)--Fuc-(1→组成，并在残基B的2位有分支t--Man-(1→。PFSV-2通过减少斑马鱼幼虫的脂质积累以及总胆固醇和甘油三酯水平表现出降血脂活性。此外，PFSV-2下调了[具体基因未给出，暂用相关基因表示]基因，并上调了[具体基因未给出，暂用相关基因表示]基因。这些发现表明PFSV-2可能是脂质调节治疗中有前景的候选物。
