Recovery of a hypolipidemic polysaccharide from artificially cultivated with an effective method.

In this study, an effective method was developed to extract the polysaccharide from (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)--Gal-(1→ and →2,6)--Man-(1→ and →2)--Fuc-(1→ and was branched of t--Man-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the , and genes and upregulated the and genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.