Martínez-Cánovas Maria José, Quesada Emilia, Martínez-Checa Fernando, Béjar Victoria
Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada, Spain.
Curr Microbiol. 2004 May;48(5):348-53. doi: 10.1007/s00284-003-4208-0.
This study was undertaken to identify exopolysaccharide-producing bacteria gathered from 18 hypersaline habitats. Phenotypic studies performed with 134 isolates revealed the majority of them to be Gram-negative rods with respiratory metabolism, belonging to the genus Halomonas. A numerical analysis of the 114 phenotypic data showed that at an 80% similarity level most of the strains (121) could be grouped into six phenotypic groups. Phenon A included 25 new isolates and the reference strain of Halomonas eurihalina, and phenon B was formed by 77 new isolates and Halomonas maura. Phenon C was also related to H. maura although to a lesser extent than strains in group B. Three phena (D, E, and F) could not be grouped with any of the reference strains and may represent new taxa; their G + C contents and DNA-DNA hybridization data corroborated this hypothesis. Results of this work proved that the most abundant halophilic species EPS producer in hypersaline habitats was H. maura, followed by H. eurihalina.
本研究旨在鉴定从18个高盐生境中采集的产胞外多糖细菌。对134株分离菌进行的表型研究表明，它们大多数是具有呼吸代谢的革兰氏阴性杆菌，属于嗜盐单胞菌属。对114个表型数据的数值分析表明，在80%的相似性水平下，大多数菌株（121株）可分为六个表型组。表型组A包括25株新分离菌和嗜盐栖嗜盐单胞菌参考菌株，表型组B由77株新分离菌和毛嗜盐单胞菌组成。表型组C也与毛嗜盐单胞菌有关，但其程度低于B组菌株。三个表型组（D、E和F）无法与任何参考菌株归为一组，可能代表新的分类单元；它们的G + C含量和DNA-DNA杂交数据证实了这一假设。这项工作的结果证明，高盐生境中最丰富的嗜盐产EPS物种是毛嗜盐单胞菌，其次是嗜盐栖嗜盐单胞菌。
