Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Biomedical Sciences, Petra University, Amman, Jordan.
Folia Microbiol (Praha). 2010 Nov;55(6):588-92. doi: 10.1007/s12223-010-0094-6. Epub 2011 Jan 21.
The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.
作为努力理解和控制杀菌剂耐受性及其对其他杀菌剂的交叉抗性和抗生素抗性的一部分，测定了铜绿假单胞菌 PAO1（P.a.）对生物杀灭剂 2-苯氧乙醇（PE）浓度增加的生长适应性。在含有逐渐增加亚最小抑菌浓度 PE 的培养基中反复传代后，P.a.表现出适应性抗性，第 10 代时 MIC 增加了两倍。抗性稳定，在无 PE 的生长培养基中连续传代 7 次后仍保持不变。该菌株对不同的杀菌剂表现出交叉抗性，并且对抗生素的敏感性增加，而这不受外排抑制剂“羰基氰化物 m-氯苯腙”的影响。适应性菌株的外膜经 SDS-PAGE 检查时显示出改变的蛋白质图谱。
