Liaoning Entry-Exit Inspection & Quarantine Bureau, Dalian 116001, China.
Folia Microbiol (Praha). 2010 Nov;55(6):607-13. doi: 10.1007/s12223-010-0098-2.
PCR and SYBR Green I real-time PCR techniques were applied to the rapid detection of Vibrio metschnikovii by designing primers based on infC (initiation factor 3) gene sequence. The specificity, sensitivity, and practical applications of the methods have been also analyzed. The methods showed high detecting specificity with no cross amplifications of other closely related and nonrelated species; they provide a simple and rapid tool for V. metschnikovii detection with high sensitivity and specificity.
PCR 和 SYBR Green I 实时 PCR 技术通过基于 infC（起始因子 3）基因序列设计引物，用于快速检测梅奇尼科夫弧菌。还分析了这些方法的特异性、敏感性和实际应用。这些方法具有很高的检测特异性，不会与其他密切相关和不相关的物种发生交叉扩增；它们为 V. metschnikovii 的检测提供了一种简单、快速、具有高灵敏度和特异性的工具。
