USDA-ARS Foreign Disease Weed Science Research Unit, U.S. Department of Agriculture, 1301 Ditto Avenue, Fort Detrick, MD 21702, USA.
Virology. 2011 Mar 30;412(1):46-54. doi: 10.1016/j.virol.2011.01.001. Epub 2011 Jan 21.
Soybean dwarf virus (SbDV), first identified as an agricultural problem in Japan, has emerged as a growing problem in the Midwestern United States. The majority of research on SbDV had been limited to four lab maintained strains from Japan. SbDV had been found in clover in the eastern United States, but these isolates rarely emerged into soybeans. These isolates were analyzed by multiplex PCR and sequencing, revealing that some were infections of both Y and D components, including a recombinant subisolate. Phylogenetic analyses for the US isolates revealed a broad diversity of SbDV, with selection pressure greater on the movement protein than the coat protein. The field isolates from the Eastern United States showed differences in symptoms, aphid transmission and host range, demonstrating that a study of field isolates is an important complement to laboratory maintained strains in understanding the biology and evolution of plant viruses.
大豆矮化病毒（SbDV）最早在日本被发现是农业问题，现已成为美国中西部地区日益严重的问题。对 SbDV 的大多数研究仅限于来自日本的四种实验室维持株系。在美国东部的三叶草中发现了 SbDV，但这些分离株很少在大豆中出现。通过多重 PCR 和测序对这些分离株进行了分析，结果表明，其中一些同时感染了 Y 和 D 成分，包括一个重组亚分离株。对美国分离株的系统发育分析显示，SbDV 具有广泛的多样性，运动蛋白受到的选择压力大于外壳蛋白。来自美国东部的田间分离株在症状、蚜虫传播和宿主范围方面存在差异，这表明对田间分离株的研究是对实验室维持株系的重要补充，有助于了解植物病毒的生物学和进化。
