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[几种检测抗红细胞同种抗体方法的比较]

[Comparison of several methods for detecting anti-erythrocyte alloantibodies].

作者信息

Bencomo A A

机构信息

Instituto de Hematología e Inmunología, Ciudad de la Habana, Cuba.

出版信息

Sangre (Barc). 1990 Aug;35(4):307-10.

PMID:2125752
Abstract

The efficacy of different methods for anti-red cell antibodies detection was assessed, variations being found in accordance with the specificity of the alloantibodies. The usefulness of enzyme tests in anti-Rh antibody detection was demonstrated, as well as that of low ionic strength saline solutions in detecting anti-Kell, anti-Duffy and anti-Kidd antibodies. Serum precipitation with 15% polyethyleneglycol 8000 previously to indirect antiglobulin test was found the most sensitive method, providing the best results in all the antibodies studied.

摘要

评估了不同方法检测抗红细胞抗体的效果,发现根据同种抗体的特异性存在差异。证明了酶试验在检测抗Rh抗体中的有用性,以及低离子强度盐溶液在检测抗Kell、抗Duffy和抗Kidd抗体中的有用性。发现在间接抗球蛋白试验之前用15%聚乙二醇8000进行血清沉淀是最敏感的方法,在所有研究的抗体中都能提供最佳结果。

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