梅迪-维斯纳病毒在印度自然感染的小型反刍动物不同器官中的分布

Distribution of maedi-visna virus in different organs of the spontaneously affected small ruminants in India.

作者信息

Valecha Sh, Yadav H S, Roopa N, Vinaykumar S D, Bhardwaj M, Singh V, Kumar P

机构信息

MVSc Student in Veterinary Pathology, Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India.

Ph.D. Student in Veterinary Pathology, Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India.

出版信息

Iran J Vet Res. 2024;25(1):41-47. doi: 10.22099/IJVR.2024.47429.6844.

DOI:10.22099/IJVR.2024.47429.6844

PMID:39156799

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11327649/

Abstract

BACKGROUND

Maedi-visna (MV) is a small ruminant lentiviral (SRLV) disease affecting sheep and goats, and causes pathological alterations in various organs including lungs, pulmonary lymph nodes, mammary glands, joints, and CNS. Present study was focused to detect the MV virus (MVV) nucleic acid and MVV p28 antigen in different organs of the spontaneously MVV affected sheep and goats.

METHODS

Total of 657 samples were collected from sheep and goats (169 blood, 136 lungs, 96 pulmonary lymph nodes, 74 brain, 54 mammary gland, 78 joints, and 50 spleen) and screened for MVV nucleic acid using nested PCR assay. Serum samples were screened for SRLV antibodies by cELISA. Immunolocalization of MVV was demonstrated by using the polyclonal antibody against p28 antigen by immunohistochemistry in lungs, lymph nodes, mammary glands, and joint tissues.

RESULTS

Out of 657 samples, 10.7% (70) were found positive for MVV. Among different organs, lungs showed highest positivity (25.7%) followed by mammary glands (14.8%), blood (9.5%), joint tissues (7.7%), brain (5.4%), and pulmonary lymph node (1.0%). SRLV antibodies were detected in 29.2% of the serum samples of both sheep and goats by cELISA. MVV p28 antigen immunostaining was observed in lungs, lymph nodes, mammary glands, and joint tissues. However, the presence of MVV p28 antigen could not be demonstrated in the brain tissues.

CONCLUSION

The highest positivity of MVV in lung tissues indicated higher predilection of the virus in the pulmonary tissue.

摘要

背景

梅迪-维斯纳病（MV）是一种影响绵羊和山羊的小反刍兽慢病毒（SRLV）疾病，会导致包括肺、肺淋巴结、乳腺、关节和中枢神经系统在内的各种器官发生病理改变。本研究旨在检测自然感染MV病毒（MVV）的绵羊和山羊不同器官中的MVV核酸和MVV p28抗原。

方法

从绵羊和山羊身上共采集了657份样本（169份血液、136份肺、96份肺淋巴结、74份脑、54份乳腺、78份关节和50份脾脏），并使用巢式PCR检测法对MVV核酸进行筛查。通过竞争ELISA（cELISA）对血清样本进行SRLV抗体筛查。使用抗p28抗原的多克隆抗体通过免疫组织化学在肺、淋巴结、乳腺和关节组织中对MVV进行免疫定位。

结果

在657份样本中，10.7%（70份）被检测出MVV呈阳性。在不同器官中，肺的阳性率最高（25.7%），其次是乳腺（14.8%）、血液（9.5%）、关节组织（7.7%）、脑（5.4%）和肺淋巴结（1.0%）。通过cELISA在29.2%的绵羊和山羊血清样本中检测到了SRLV抗体。在肺、淋巴结、乳腺和关节组织中观察到了MVV p28抗原免疫染色。然而，在脑组织中未检测到MVV p28抗原。