Dina D, Penhoet E E
J Virol. 1978 Sep;27(3):768-75. doi: 10.1128/JVI.27.3.768-775.1978.
NIH 3T3 cells infected with Moloney murine sarcoma virus (murine leukemia virus) produce virions which contain about 99% murine sarcoma virus RNA and 1% murine leukemia virus RNA. This report describes experiments which measured intracellular concentrations of proviral DNA and RNA transcripts for each of the viruses. We found that three to four copies of proviral DNA from each virus were integrated into the cellular DNA. Measurements of RNA specific for each of the genomes by hybridization to specific cDNA reagents revealed a 10- to 15-fold difference in concentration in both nuclear and polysomal RNA fractions, with murine sarcoma virus RNA predominating in both cases. Unless there are major differences in stability between the two viral RNAs, our results suggest that transcriptional control is responsible for much of the difference in final levels of virus synthesis.
感染莫洛尼鼠肉瘤病毒（鼠白血病病毒）的NIH 3T3细胞产生的病毒粒子含有约99%的鼠肉瘤病毒RNA和1%的鼠白血病病毒RNA。本报告描述了测量每种病毒前病毒DNA和RNA转录本细胞内浓度的实验。我们发现，每种病毒的三到四个前病毒DNA拷贝整合到了细胞DNA中。通过与特异性cDNA试剂杂交来测量每个基因组特异性的RNA，结果显示在核RNA和多聚核糖体RNA组分中浓度存在10到15倍的差异，在这两种情况下都是鼠肉瘤病毒RNA占主导。除非两种病毒RNA在稳定性上存在重大差异，我们的结果表明转录控制是病毒合成最终水平差异的主要原因。
