劳氏肉瘤病毒特异性RNA的体外合成是由一种依赖于DNA的RNA聚合酶催化的。
In vitro synthesis of Rous sarcoma virus-specific RNA is catalyzed by a DNA-dependent RNA polymerase.
作者信息
Rymo L, Parsons J T, Coffin J M, Weissmann C
出版信息
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2782-6. doi: 10.1073/pnas.71.7.2782.
Abstract
Synthesis of Rous sarcoma virus RNA was examined in vitro with a new assay for radioactive virus-specific RNA. Nuclei from infected and uninfected cells were incubated with ribonucleoside [alpha-(32)P]triphosphates, Mn(++), Mg(++) and (NH(4))(2)SO(4). Incorporation into total and viral RNA proceeded with similar kinetics for up to 25 min at 37 degrees . About 0.5% of the RNA synthesized by the infected system was scored as virus-specific, compared to 0.03% of the RNA from the uninfected system and 0.005% of the RNA synthesized by monkey kidney cell nuclei. Preincubation with DNase or actinomycin D completely suppressed total and virus-specific RNA synthesis. alpha-Amanitin, a specific inhibitor of eukaryotic RNA polymerase II, completely inhibited virus-specific RNA synthesis, while reducing total RNA synthesis by only 50%. We conclude that tumor virus-specific RNA is synthesized on a DNA template, most probably by the host's RNA polymerase II.
摘要
采用一种新的放射性病毒特异性RNA检测方法，对劳氏肉瘤病毒RNA的合成进行了体外研究。将感染细胞和未感染细胞的细胞核与核糖核苷[α-(32)P]三磷酸、Mn(++)、Mg(++)和(NH4)2SO4一起孵育。在37℃下，掺入总RNA和病毒RNA的过程在长达25分钟内具有相似的动力学。与未感染系统合成的RNA的0.03%以及猴肾细胞核合成的RNA的0.005%相比，感染系统合成的RNA中约0.5%被视为病毒特异性的。用DNA酶或放线菌素D预孵育可完全抑制总RNA和病毒特异性RNA的合成。α-鹅膏蕈碱是真核RNA聚合酶II的特异性抑制剂，它完全抑制病毒特异性RNA的合成，同时仅使总RNA合成减少50%。我们得出结论，肿瘤病毒特异性RNA是在DNA模板上合成的，很可能是由宿主的RNA聚合酶II合成的。
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Proc Natl Acad Sci U S A. 1974 Jul;71(7):2782-6. doi: 10.1073/pnas.71.7.2782.
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