Kotake T, Saiki S, Kinouchi T, Shiku H, Nakayama E
Department of Urology, Center for Adult Diseases, Osaka.
Jpn J Cancer Res. 1990 Dec;81(12):1198-201. doi: 10.1111/j.1349-7006.1990.tb02677.x.
Expression of the c-myc gene product in urinary bladder cancer was investigated by immunohistochemical staining with anti-c-myc monoclonal antibody (mAb) MYC-1. Positive staining was observed in the cytoplasm, but not in the nucleus in tissues fixed with 10% formalin. On the other hand, positive staining was localized in the nucleus in cryopreserved tissues. Of 34 cryopreserved specimens examined, positive staining with MYC-1 mAb was observed in 1 of 12 (8.3%) of grade 1 (G1), 12 of 15 (80%) of G2 and 6 of 7 (86%) of G3. Positive staining with Ki-67 mAb was observed in 2 of 12 (17%) of G1, 12 of 15 (80%) of G2, and 6 of 7 (86%) of G3. These results suggest that tumors with higher nuclear pleomorphism contain more proliferating cells.
采用抗c-myc单克隆抗体（mAb）MYC-1进行免疫组织化学染色，研究c-myc基因产物在膀胱癌中的表达情况。在用10%福尔马林固定的组织中，阳性染色见于细胞质而非细胞核。另一方面，在冷冻保存的组织中，阳性染色定位于细胞核。在检查的34个冷冻保存标本中，1级（G1）的12个中有1个（8.3%）、G2的15个中有12个（80%）、G3的7个中有6个（86%）观察到MYC-1 mAb阳性染色。Ki-67 mAb阳性染色在G1的12个中有2个（17%）、G2的15个中有12个（80%）、G3的7个中有6个（86%）观察到。这些结果表明，核多形性较高的肿瘤含有更多增殖细胞。
