Stewart J, Evan G, Watson J, Sikora K
Br J Cancer. 1986 Jan;53(1):1-6. doi: 10.1038/bjc.1986.1.
The c-myc oncogene has been implicated in the processes of normal cell proliferation and differentiation. Elevated levels of c-myc mRNA and its gene product (p62c-myc), have been detected in a variety of solid tumours and cultured cel lines. Its precise role in normal cell function and in neoplastic transformation and progression has yet to be elucidated. We have used a monoclonal antibody, raised by peptide immunisation, to determine the distribution by immunoperoxidase staining of the c-myc oncogene product in archival specimens of colonic polyps and carcinomas. Samples from 42 patients with colon carcinoma, 24 with benign polyps and 15 normal colon biopsies were examined. Normal colon revealed maximum staining in the mid-zone of the crypts, corresponding to the zone of differentiation and maturation. The staining was predominantly cytoplasmic. Adenomatous polyps revealed the most intense pattern of staining in areas of dysplastic change. Colonic tumours showed a wide range of staining. Well differentiated tumours contained more cytoplasmic p62c-myc than poorly differentiated tumours. These findings suggest that the c-myc oncogene product may play an important role in the evolution of colonic neoplasia.
c-myc癌基因与正常细胞增殖和分化过程有关。在多种实体瘤和培养细胞系中已检测到c-myc mRNA及其基因产物（p62c-myc）水平升高。其在正常细胞功能以及肿瘤转化和进展中的精确作用尚待阐明。我们使用了一种通过肽免疫产生的单克隆抗体，通过免疫过氧化物酶染色来确定c-myc癌基因产物在结肠息肉和癌存档标本中的分布。检查了42例结肠癌患者、24例良性息肉患者和15例正常结肠活检的样本。正常结肠在隐窝中区显示出最强染色，对应于分化和成熟区。染色主要位于细胞质。腺瘤性息肉在发育异常改变区域显示出最强的染色模式。结肠肿瘤显示出广泛的染色。高分化肿瘤比低分化肿瘤含有更多的细胞质p62c-myc。这些发现表明c-myc癌基因产物可能在结肠肿瘤形成过程中起重要作用。
