果蝇蛋白酶体（MCP）在早期发育过程中的细胞特异性积累。
Cell-specific accumulation of Drosophila proteasomes (MCP) during early development.
作者信息
Klein U, Gernold M, Kloetzel P M
机构信息
Zentrum für Molekulare Biologie/Molekulare Genetik, University of Heidelberg, Federal Republic of Germany.
出版信息
J Cell Biol. 1990 Dec;111(6 Pt 1):2275-82. doi: 10.1083/jcb.111.6.2275.
Abstract
The proteasome (MCP) is a high relative molecular mass multicatalytic proteinase complex composed of nonidentical protein subunits. We have investigated the cellular distribution of the enzyme complex during Drosophila embryogenesis using the proteasome specific antibodies N19-35 and N19-28 for immunocytology. Antibody staining of whole-mount embryos shows that during embryogenesis proteasomes are present in proliferating cells and that their accumulation and turnover is differentially regulated. Our data suggest that the proteasome may serve different proteolytic processes and that the enzyme may be involved in cell-specific proteolytic events required for cell proliferation and morphogenesis during early Drosophila development.
摘要
蛋白酶体（MCP）是一种由不同蛋白质亚基组成的高分子量多催化蛋白酶复合物。我们使用蛋白酶体特异性抗体N19 - 35和N19 - 28进行免疫细胞化学研究，以探究果蝇胚胎发育过程中该酶复合物的细胞分布。对整装胚胎的抗体染色显示，在胚胎发育过程中，蛋白酶体存在于增殖细胞中，并且它们的积累和周转受到不同的调节。我们的数据表明，蛋白酶体可能参与不同的蛋白水解过程，并且该酶可能参与果蝇早期发育过程中细胞增殖和形态发生所需的细胞特异性蛋白水解事件。
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