Pasten Consuelo, Ortiz-Pineda Pablo A, García-Arrarás José E
Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan, Puerto Rico 00931.
Genesis. 2012 Apr;50(4):350-65. doi: 10.1002/dvg.20803. Epub 2012 Jan 6.
The ubiquitin proteasome system (UPS) is the main proteolytic system of cells. Recent evidence suggests that the UPS plays a regulatory role in regeneration processes. Here, we explore the possibility that the UPS is involved during intestinal regeneration of the sea cucumber Holothuria glaberrima. These organisms can regenerate most of their digestive tract following a process of evisceration. Initially, we identified components of H. glaberrima UPS, including sequences for Rpn10, β3, and ubiquitin-RPL40. Predicted proteins from the mRNA sequences showed high degree of conservation that ranged from 60% (Rpn10) to 98% (Ub-RPL40). Microarrays and RT-PCR experiments showed that these genes were upregulated during intestinal regeneration. In addition, we demonstrated expression of alpha 20S proteasome subunits and ubiquitinated proteins during intestinal regeneration and detected them in the epithelium and connective tissue of the regenerating intestine. Finally, the intestinal regeneration was altered in animals treated with MG132, a proteasome inhibitor. These findings support our contention that proteasomes are playing an important role during intestinal regeneration.
泛素蛋白酶体系统（UPS）是细胞的主要蛋白水解系统。最近的证据表明，UPS在再生过程中发挥调节作用。在此，我们探讨了UPS参与光滑海参肠道再生过程的可能性。这些生物在去内脏过程后能够再生其大部分消化道。最初，我们鉴定了光滑海参UPS的组成成分，包括Rpn10、β3和泛素-RPL40的序列。从mRNA序列预测的蛋白质显示出高度的保守性，范围从60%（Rpn10）到98%（Ub-RPL40）。微阵列和RT-PCR实验表明，这些基因在肠道再生过程中上调。此外，我们证明了α20S蛋白酶体亚基和泛素化蛋白在肠道再生过程中的表达，并在再生肠道的上皮和结缔组织中检测到它们。最后，用蛋白酶体抑制剂MG132处理的动物的肠道再生发生了改变。这些发现支持了我们的观点，即蛋白酶体在肠道再生过程中发挥着重要作用。
