Beucler I, Caces E, Camare R, Dachet C, Fruchart J C, Gambert P, Girault A, Leglise D, Legrand A, Mainard F
ARCOL (Comité Français de Coordination des Recherches sur l'Athérosclérose et le Cholestérol, Saint-Maur.
Ann Biol Clin (Paris). 1990;48(7):443-7.
The proliferation of commercial immunoassays for the determination of apolipoproteins AI and B as parameters of risk for coronary heart disease and the discordance between the values obtained with the systems have made more urgent than ever the need for standardization of these assays. The authors report here the results of a collaborative study on the standardization of the values between the "Comité Française de Coordination des Recherches sur l'Athérosclérose et le Cholestérol (ARCOL)" and 13 Companies providing 15 different analytical systems. The results show that standardized measurements are not possible using a "consensus international standard" and that the major problem is related to the enormous variability of the analytical systems. In contrast, the present study demonstrates that six different serum pools containing increasing amounts of apolipoproteins AI and B can be used to generate a common reference curve which permits calibration of all immunoassays and standardization of results.
用于测定载脂蛋白AI和B作为冠心病风险参数的商业免疫测定法的激增，以及不同系统所获值之间的不一致，使得这些测定法的标准化需求比以往任何时候都更加迫切。作者在此报告了一项合作研究的结果，该研究涉及“法国动脉粥样硬化与胆固醇研究协调委员会（ARCOL）”与提供15种不同分析系统的13家公司之间的值标准化。结果表明，使用“国际共识标准”无法实现标准化测量，主要问题与分析系统的巨大变异性有关。相比之下，本研究表明，含有不同量载脂蛋白AI和B的六个不同血清池可用于生成一条通用参考曲线，该曲线可用于校准所有免疫测定并实现结果的标准化。
