Steinmetz J, Caces E, Couderc R, Beucler I, Legrand A, Henny J
Laboratoire du Centre de médecine preventive, Vandoeuvre-lès-Nancy.
Ann Biol Clin (Paris). 1997 Sep-Oct;55(5):451-4.
The utilization of two WHO reference materials, liquid and lyophilized, permitted international standardization of apolipoprotein measurements. We report here the results of a collaborative study between Arcol, SFBC and SFRL in order to establish reference ranges for apo A1 and B on nine standardized systems. A population of 1027 men and women supposed healthy, 4 to 60 year old, have been selected in two Centers for Preventive Medicine. The serum samples were aliquoted frozen at -20 degrees C the day of sampling and analysed by the manufacturers with IFCC standardized calibrants. A specific quality control was performed using a frozen pool of sera. For apo A1, the centile 2.5 of the reference population varies from 1.04 to 1.16 g/l. The range values for the centile 97.5 varies from 1.87 to 2.24 g/l. For apoB, the centile 2.5 varies from 0.43 to 0.57 g/l, and the centile 97.5 from 1.30 to 1.39 g/l. Only one system has a problem of dispersion with an upper limit equal to 1.20 g/l. These results improve that international standardization allowed actually a good comparability of the results, especially for apoB.
使用两种世界卫生组织参考物质（液体和冻干形式）实现了载脂蛋白测量的国际标准化。我们在此报告Arcol、SFBC和SFRL之间的一项合作研究结果，以便在九个标准化系统上确定载脂蛋白A1和B的参考范围。在两个预防医学中心选取了1027名年龄在4至60岁之间的健康男性和女性。血清样本在采样当天分装并在-20℃冷冻，由制造商使用国际临床化学和检验医学联合会（IFCC）标准化校准物进行分析。使用血清冷冻混合样本进行了特定的质量控制。对于载脂蛋白A1，参考人群的第2.5百分位数在1.04至1.16 g/l之间变化。第97.5百分位数的范围值在1.87至2.24 g/l之间变化。对于载脂蛋白B，第2.5百分位数在0.43至0.57 g/l之间变化，第97.5百分位数在1.30至1.39 g/l之间变化。只有一个系统存在离散问题，上限为1.20 g/l。这些结果表明，国际标准化实际上使结果具有良好的可比性，尤其是对于载脂蛋白B。
