Silva L C, Borojevic R, Mourão P A
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
Int J Exp Pathol. 1990 Dec;71(6):845-56.
Sulphated glycosaminoglycans were isolated from schistosome-induced hepatic granuloma and from the pericellular, intracellular and extracellular compartments of two murine cell lines derived from granulomas: the primary cell line GR, and the permanent cell line GRX, established spontaneously from GR. The glycosaminoglycans composition in the whole granuloma was similar to that observed in the intracellular and extracellular compartments of GR cells. This result suggests that GR cells may be the major cell population involved in the synthesis and accumulation of glycosaminoglycans in the granulomas, and play an important role in the process of hepatic fibrosis. The conversion of the primary cell line GR into the established GRX cells did not modify the ratios that prevail among different glycosaminoglycans of the cell surface. However, it decreased the synthesis and secretion of glycosaminoglycans, reduced the proportion of iduronic acid units in the chondroitin sulphate, and increased the proportion of heparan sulphate in intracellular and extracellular pools. These characteristics of the GRX cells are similar to those observed in long-term cultures of smooth-muscle cells. In agreement with the general phenomenon of progressive de-differentiation during in-vitro culture of primary cell lines, these data indicate that the connective tissue cells of liver may belong to the myofibroblastic cell lineage.
从血吸虫诱导的肝肉芽肿以及源自肉芽肿的两种小鼠细胞系（原代细胞系GR和从GR自发建立的永久细胞系GRX）的细胞周围、细胞内和细胞外区室中分离出硫酸化糖胺聚糖。整个肉芽肿中的糖胺聚糖组成与GR细胞的细胞内和细胞外区室中观察到的相似。这一结果表明，GR细胞可能是肉芽肿中参与糖胺聚糖合成和积累的主要细胞群体，并在肝纤维化过程中发挥重要作用。原代细胞系GR向已建立的GRX细胞的转化并未改变细胞表面不同糖胺聚糖之间占主导的比例。然而，它减少了糖胺聚糖的合成和分泌，降低了硫酸软骨素中艾杜糖醛酸单元的比例，并增加了细胞内和细胞外池中硫酸乙酰肝素的比例。GRX细胞的这些特征与平滑肌细胞长期培养中观察到的特征相似。与原代细胞系体外培养过程中逐渐去分化的普遍现象一致，这些数据表明肝脏的结缔组织细胞可能属于肌成纤维细胞谱系。
