Monteiro A N, Geremias A T, Borojevic R
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
Braz J Med Biol Res. 1995 Apr;28(4):457-66.
GR primary cells cultures were isolated from hepatic granulomas induced in C3H mice livers by Schistosoma mansoni infection; the GRX continuous cell line was derived from GR cells after long-term culture and a progressive drift towards a rapidly proliferating cell population. These cells were analyzed and compared in terms of their clonal heterogeneity. Clones were classified on the basis of cell substrate, cell-cell adhesion (growth morphology of the clone) and fat droplet accumulation. GR cells were composed of two slow-growing clone types, while GRX cells gave rise to clones with several phenotypes, including the two found in the GR cells. The overall proportion of different clones in the GRX cell population was stable in long-term cultures, as well as after recloning of the highly proliferating, but not the slowly proliferating, clones. We propose that the slow-growing clones are maintained in the overall population by continuous contribution of new slow-growing cells from the rapidly growing ones. The slow-growing clones may represent the basal population of liver connective tissue cells that can be mobilized into injured tissues and that are involved in tissue repair. The highly proliferating clones with a broad capacity of phenotype expression that arise after long-term growth stimulation of the local cell population may represent the hypertrophic connective tissue cells, such as those observed in progressive fibrotic reactions associated with chronic liver tissue inflammation.
糖皮质激素受体（GR）原代细胞培养物是从曼氏血吸虫感染诱导的C3H小鼠肝脏中的肝肉芽肿中分离出来的；GRX连续细胞系是由GR细胞经过长期培养并逐渐向快速增殖细胞群体漂移后获得的。对这些细胞的克隆异质性进行了分析和比较。根据细胞底物、细胞间粘附（克隆的生长形态）和脂滴积累对克隆进行分类。GR细胞由两种生长缓慢的克隆类型组成，而GRX细胞产生了具有多种表型的克隆，包括在GR细胞中发现的两种表型。在长期培养中，以及在对高度增殖但非缓慢增殖的克隆进行再克隆后，GRX细胞群体中不同克隆的总体比例是稳定的。我们提出，生长缓慢的克隆通过快速生长的克隆持续贡献新的生长缓慢的细胞而在总体群体中得以维持。生长缓慢的克隆可能代表肝脏结缔组织细胞的基础群体，这些细胞可以被动员到受损组织中并参与组织修复。在局部细胞群体长期生长刺激后出现的具有广泛表型表达能力的高度增殖克隆可能代表肥大的结缔组织细胞，例如在与慢性肝组织炎症相关的进行性纤维化反应中观察到的那些细胞。
