FOI, Cementvägen 20, SE-901 82 Umeå, Sweden.
Small. 2011 Feb 18;7(4):514-23. doi: 10.1002/smll.201001832. Epub 2011 Jan 24.
The cellular uptake and distribution of five types of well-characterized anatase and rutile TiO(2) nanoparticles (NPs) in A549 lung epithelial cells is reported. Static light scattering (SLS), in-vitro Raman microspectroscopy (μ-Raman) and transmission electron spectroscopy (TEM) reveal an intimate correlation between the intrinsic physicochemical properties of the NPs, particle agglomeration, and cellular NP uptake. It is shown that μ-Raman facilitates chemical-, polymorph-, and size-specific discrimination of endosomal-particle cell uptake and the retention of particles in the vicinity of organelles, including the cell nucleus, which quantitatively correlates with TEM and SLS data. Depth-profiling μ-Raman coupled with hyperspectral data analysis confirms the location of the NPs in the cells and shows that the NPs induce modifications of the biological matrix. NP uptake is found to be kinetically activated and strongly dependent on the hard agglomeration size-not the primary particle size-which quantitatively agrees with the measured intracellular oxidative stress. Pro-inflammatory responses are also found to be sensitive to primary particle size.
本文报道了五种经过充分表征的锐钛矿型和金红石型 TiO(2)纳米粒子（NPs）在 A549 肺上皮细胞中的细胞摄取和分布情况。静态光散射（SLS）、体外拉曼微光谱（μ-Raman）和透射电子光谱（TEM）揭示了 NPs 的固有物理化学性质、颗粒聚集和细胞内 NP 摄取之间的密切关系。研究表明，μ-Raman 有利于对内涵体颗粒细胞摄取的化学、多晶型和大小特异性的区分，以及颗粒在细胞器附近（包括细胞核）的保留，这与 TEM 和 SLS 数据定量相关。深度分布 μ-Raman 与高光谱数据分析相结合，证实了 NPs 在细胞中的位置，并表明 NPs 诱导了生物基质的修饰。NP 摄取被发现是动力学激活的，并且强烈依赖于硬团聚体的大小，而不是初级粒子的大小，这与测量的细胞内氧化应激定量一致。还发现促炎反应对初级粒子的大小敏感。
