INSERM, U955, Créteil F-94000, France.
Toxicol In Vitro. 2012 Feb;26(1):57-66. doi: 10.1016/j.tiv.2011.10.010. Epub 2011 Oct 19.
Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO(2)), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO(2) MNPs had similar accumulation patterns. However, TiO(2) aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.
几项研究表明，制造纳米颗粒（MNPs）引起的生物反应可能与其在细胞内的积累有关。然而，MNP 的内化尚未得到充分描述。因此，本研究的目的是比较三种不同 MNPs（两种由炭黑（CB）制成，一种由二氧化钛（TiO2）制成）在 16HBE 支气管上皮细胞和 MRC5 成纤维细胞中的细胞内摄取。透射电子显微镜用于评估细胞内的积累。分析了时间和剂量关系后的不同参数：MNPs 在细胞中的定位、积累 MNPs 的细胞百分比、细胞中聚集的 MNPs 数量以及细胞中 MNP 聚集的大小。结果表明，MNPs 在 16HBE 细胞和 MRC5 成纤维细胞中广泛且快速积累。此外，MNPs 主要以细胞溶质小泡中的聚集体形式积累，而不存在于线粒体或核中。CB 和 TiO2 MNPs 具有相似的积累模式。然而，TiO2 聚集体的尺寸大于 CB 聚集体。细胞内 MNP 积累与细胞毒性无关。这些结果表明，MNP 的细胞摄取是各种细胞类型中普遍存在的现象。
