NHC Key Laboratory of Carcinogenesis and Human Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410000, China.
Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha 410000, China.
Molecules. 2023 Feb 21;28(5):2034. doi: 10.3390/molecules28052034.
Semiconductiong polymer nanoparticles (Pdots) have a wide range of applications in biomedical fields including biomolecular probes, tumor imaging, and therapy. However, there are few systematic studies on the biological effects and biocompatibility of Pdots in vitro and in vivo. The physicochemical properties of Pdots, such as surface modification, are very important in biomedical applications. Focusing on the central issue of the biological effects of Pdots, we systematically investigated the biological effects and biocompatibility of Pdots with different surface modifications and revealed the interactions between Pdots and organisms at the cellular and animal levels. The surfaces of Pdots were modified with different functional groups, including thiol, carboxyl, and amino groups, named Pdots@SH, Pdots@COOH, and Pdots@NH, respectively. Extracellular studies showed that the modification of sulfhydryl, carboxyl, and amino groups had no significant effect on the physicochemical properties of Pdots, except that the amino modification affected the stability of Pdots to a certain extent. At the cellular level, Pdots@NH reduced cellular uptake capacity and increased cytotoxicity due to their instability in solution. At the in vivo level, the body circulation and metabolic clearance of Pdots@SH and Pdots@COOH were superior to those of Pdots@NH. The four kinds of Pdots had no obvious effect on the blood indexes of mice and histopathological lesions in the main tissues and organs. This study provides important data for the biological effects and safety assessment of Pdots with different surface modifications, which pave the way for their potential biomedical applications.
半导体聚合物纳米粒子（Pdots）在生物医学领域有广泛的应用，包括生物分子探针、肿瘤成像和治疗。然而，对于 Pdots 在体外和体内的生物效应和生物相容性，仅有少数系统研究。Pdots 的物理化学性质，如表面修饰，在生物医学应用中非常重要。我们聚焦于 Pdots 的生物效应这一核心问题，系统地研究了具有不同表面修饰的 Pdots 的生物效应和生物相容性，并揭示了 Pdots 与细胞和动物水平的生物体之间的相互作用。Pdots 的表面用不同的官能团进行了修饰，包括巯基、羧基和氨基，分别命名为 Pdots@SH、Pdots@COOH 和 Pdots@NH。细胞外研究表明，巯基、羧基和氨基的修饰除了在一定程度上影响 Pdots 的稳定性外，对其物理化学性质没有显著影响。在细胞水平上，由于在溶液中不稳定，Pdots@NH 降低了细胞摄取能力并增加了细胞毒性。在体内水平上，Pdots@SH 和 Pdots@COOH 的体循环和代谢清除能力优于 Pdots@NH。这四种 Pdots 对小鼠的血液指标和主要组织和器官的组织病理学病变没有明显影响。这项研究为具有不同表面修饰的 Pdots 的生物效应和安全性评估提供了重要数据，为它们在潜在的生物医学应用中铺平了道路。
