Academic Geriatric Medicine, University of Southampton, Southampton General Hospital, Southampton, UK.
J Nutr Health Aging. 2011 Jan;15(1):10-5. doi: 10.1007/s12603-011-0006-8.
Sarcopenia, the loss of muscle mass and strength, is associated adversely with disability, morbidity and mortality. Epidemiological findings suggest influences operating across the life course may be important. Our aim was to ascertain the feasibility and acceptability of obtaining muscle tissue from healthy older people in order to ultimately identify cellular mechanisms underlying life course influences on sarcopenia.
105 men with documented birth weight consented to detailed assessment of muscle mass and strength, and a biopsy of the vastus lateralis using the Weil-Blakesley conchotome. Acceptability was ascertained by questionnaire and a 100 mm pain Visual Analogue Scale (VAS). 100 mm indicated severe pain.
Muscle biopsy was successfully carried out in 102 out of 105 participants, mean yield 107 mg (range 20-290 mg). There were no serious wound complications. Ninety-three participants completed feedback questionnaires. The median pain VAS score during the procedure was 7 mm (Interquartile range [IQR] 1-34), 4 mm (IQR 0-16) one day after the procedure and 1mm (IQR 0-4) 7 days after the procedure. 60 (65%) participants were back to their normal levels of activity one day after the procedure. 85 (91%) found this procedure acceptable and would have the procedure again.
Muscle biopsy using a Weil-Blakesley conchotome is both feasible and acceptable in community dwelling older men participating in epidemiological research. The excellent yield of biopsy tissue will allow morphological and molecular studies of muscle to be integrated into an epidemiological study facilitating investigation of the mechanisms underpinning sarcopenia that could potentially be altered by life course influences.
肌肉减少症是肌肉质量和力量的丧失，与残疾、发病率和死亡率呈负相关。 流行病学研究结果表明，整个生命过程中的各种影响可能很重要。 我们的目的是确定从健康老年人中获取肌肉组织的可行性和可接受性，以便最终确定生命过程对肌肉减少症影响的细胞机制。
105 名男性参与者的出生体重均有记录，他们同意接受详细的肌肉质量和力量评估，并使用 Weil-Blakesley 海螺刀对股外侧肌进行活检。 通过问卷调查和 100 毫米疼痛视觉模拟量表（VAS）来确定可接受性。 100 毫米表示严重疼痛。
105 名参与者中有 102 名成功进行了肌肉活检，平均产量为 107 毫克（范围为 20-290 毫克）。 没有严重的伤口并发症。93 名参与者完成了反馈问卷。 手术过程中的中位数疼痛 VAS 评分为 7 毫米（四分位距 [IQR] 1-34），术后 1 天为 4 毫米（IQR 0-16），术后 7 天为 1 毫米（IQR 0-4）。 术后一天，60 名（65%）参与者恢复正常活动水平。 85 名（91%）参与者认为该过程是可以接受的，并愿意再次进行该过程。
在参与流行病学研究的社区居住的老年男性中，使用 Weil-Blakesley 海螺刀进行肌肉活检既可行又可接受。 活检组织的高产量将允许对肌肉进行形态和分子研究，从而将其纳入流行病学研究中，从而有助于研究潜在可能因生命过程影响而改变的肌肉减少症的机制。
