Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China.
Anim Sci J. 2011 Feb;82(1):57-61. doi: 10.1111/j.1740-0929.2010.00813.x. Epub 2010 Oct 6.
The inhibin β(B) (INHBB) gene was studied as a candidate gene for the prolificacy of Small Tail Han and Hu sheep. According to the sequence of exon 1 and 2 of bovine INHBB gene, six pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and 2 of INHBB gene in both high (Small Tail Han and Hu sheep) and low prolificacy breeds (Dorset, Texel and German Mutton Merino sheep) by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Three pairs of primers (primers 1-1, 1-2 and 1-3) were used to amplify the exon 1, and others (primers 2-1, 2-2 and 2-3) to the exon 2. Only the products amplified by primer 2-3 displayed polymorphism. For primer 2-3, three genotypes (AA, AB and BB) were detected in Hu sheep and only AA genotype in other breeds. In Hu sheep, frequency of AA, AB and BB genotypes was 0.636, 0.046 and 0.318, respectively. Sequencing revealed 276A > G mutation (based on the amplification region of primer 2-3) which did not cause any amino acid change because it lay in the 3' untranslated region. The ewes with genotype BB had 0.58 (P < 0.01) lambs more than those with AA in Hu sheep.
抑制素β亚基（INHBB）基因被作为一个候选基因，用于研究小尾寒羊和湖羊的多胎性。根据牛 INHBB 基因外显子 1 和 2 的序列，设计了 6 对引物，通过聚合酶链反应-单链构象多态性（PCR-SSCP）检测 INHBB 基因外显子 1 和 2 的单核苷酸多态性，分别在高繁殖力品种（小尾寒羊和湖羊）和低繁殖力品种（杜泊羊、特克赛尔羊和德国肉用美利奴羊）中。三对引物（引物 1-1、1-2 和 1-3）用于扩增外显子 1，其他三对引物（引物 2-1、2-2 和 2-3）用于扩增外显子 2。只有用引物 2-3 扩增的产物显示出多态性。对于引物 2-3，在湖羊中检测到 3 种基因型（AA、AB 和 BB），而在其他品种中仅检测到 AA 基因型。在湖羊中，AA、AB 和 BB 基因型的频率分别为 0.636、0.046 和 0.318。测序显示 276A > G 突变（基于引物 2-3 的扩增区域）没有引起任何氨基酸变化，因为它位于 3'非翻译区。在湖羊中，基因型为 BB 的母羊比基因型为 AA 的母羊多产 0.58 只羔羊（P < 0.01）。
