Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
Mol Biol Rep. 2012 Apr;39(4):3721-5. doi: 10.1007/s11033-011-1147-x. Epub 2011 Jul 3.
Single nucleotide polymorphisms of 5' regulatory region of follicle-stimulating hormone receptor (FSHR) gene were detected in two high prolificacy sheep breeds (Small Tail Han and Hu sheep) and two low prolificacy sheep breeds (Corriedale and Chinese Merino sheep) by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The results indicated that there were three genotypes (AA, AB and BB) detected by primer 1 in Hu sheep while only one genotype (AA) in other three sheep breeds, and frequencies of AA, AB and BB genotypes in Hu sheep were 0.700, 0.225 and 0.075, respectively. There were three genotypes (EE, EF and EG) detected by primer 3 in Small Tail Han sheep while only EE genotype occurred in other three sheep breeds, and frequencies of EE, EF and EG genotypes in Small Tail Han sheep were 0.775, 0.200 and 0.025, respectively. No polymorphism was detected in four sheep breeds by primer 2 and primer 4. The sequencing results showed that there were two nucleotide mutations (g. -681T>C and g. -629C>T) in genotype BB compared with AA for primer 1. As for primer 3, two mutations (g. -197G>A and g. -98T>C) in genotype EF compared with EE and two mutations (g. -200G>A and g. -197G>A) in genotype EG compared with EE. The heterozygous ewes with EG or EF had 0.89 (P < 0.05) or 0.42 (P < 0.05) lambs more than homozygous ewes (EE genotype) in Small Tail Han sheep, respectively, while there was no significant difference on litter size between EG and EF ewes.
采用聚合酶链反应-单链构象多态性（PCR-SSCP）技术，对小尾寒羊和湖羊两个高产绒绵羊品种及 4 个低产绒绵羊品种（陶赛特羊、无角道赛特羊、中国美利奴羊和新疆细毛羊）的促卵泡素受体（FSHR）基因 5′调控区的单核苷酸多态性进行检测。结果表明，引物 1 在湖羊中共检测到 3 种基因型（AA、AB 和 BB），而在其他 3 个绵羊品种中仅检测到 1 种基因型（AA），湖羊 AA、AB 和 BB 基因型的频率分别为 0.700、0.225 和 0.075。引物 3 在小尾寒羊中共检测到 3 种基因型（EE、EF 和 EG），而在其他 3 个绵羊品种中仅检测到 EE 基因型，小尾寒羊 EE、EF 和 EG 基因型的频率分别为 0.775、0.200 和 0.025。引物 2 和引物 4 未在 4 个绵羊品种中检测到多态性。测序结果显示，与 AA 相比，引物 1 中 BB 基因型有 2 个核苷酸突变（g.-681T>C 和 g.-629C>T）。对于引物 3，EF 基因型与 EE 相比有 2 个突变（g.-197G>A 和 g.-98T>C），EG 基因型与 EE 相比有 2 个突变（g.-200G>A 和 g.-197G>A）。小尾寒羊中，EG 或 EF 杂合母羊的产羔数比 EE 纯合母羊分别多 0.89（P<0.05）和 0.42（P<0.05），而 EG 和 EF 母羊之间的产羔数没有显著差异。
