Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
Mol Biol Rep. 2012 Oct;39(10):9801-7. doi: 10.1007/s11033-012-1846-y. Epub 2012 Jun 24.
The insulin-like growth factor 1 (IGF1) gene was studied as a candidate gene for high prolificacy in sheep. Polymorphisms of 5' regulatory region and all four exons of IGF1 gene were detected in Small Tail Han (n = 277), Hu (n = 58), Texel (n = 48) and Dorset (n = 46) sheep by PCR-RFLP and PCR-SSCP analysis. A microsatellite polymorphic site and a restriction fragment length polymorphism were shown in the 5' regulatory region of IGF1 gene. The ewes with genotype 123/123 bp had 0.81 (P < 0.05) or 1.03 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp, the ewes with genotype 123/125 bp had 0.46 (P < 0.05) or 0.68 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp. In addition, there were two mutations (C1511G and A1513G) in 5' regulatory region of IGF1 gene. The ewes with genotype BB or AB had 0.96 (P < 0.05) or 0.38 (P < 0.05) lambs more than those with genotype AA, but there were no significant differences between BB and AB genotypes (P > 0.05) in Small Tail Han sheep. These results preliminarily indicated that these polymorphisms of IGF1 gene could be used in molecular marker-assisted selection for sheep breeding programs.
胰岛素样生长因子 1（IGF1）基因被研究为绵羊高繁殖力的候选基因。通过 PCR-RFLP 和 PCR-SSCP 分析，在小尾寒羊（n = 277）、湖羊（n = 58）、特克赛尔羊（n = 48）和杜洛赛特羊（n = 46）中检测到 IGF1 基因 5'调控区和所有四个外显子的多态性。在 IGF1 基因 5'调控区显示出一个微卫星多态性位点和一个限制性片段长度多态性。基因型为 123/123 bp 的母羊比基因型为 125/125 bp 或 125/127 bp 的母羊多产 0.81 只（P < 0.05）或 1.03 只（P < 0.01）羔羊，基因型为 123/125 bp 的母羊比基因型为 125/125 bp 或 125/127 bp 的母羊多产 0.46 只（P < 0.05）或 0.68 只（P < 0.01）羔羊。此外，在 IGF1 基因 5'调控区有两个突变（C1511G 和 A1513G）。基因型为 BB 或 AB 的母羊比基因型为 AA 的母羊多产 0.96 只（P < 0.05）或 0.38 只（P < 0.05）羔羊，但在小尾寒羊中 BB 和 AB 基因型之间没有显著差异（P > 0.05）。这些结果初步表明，IGF1 基因的这些多态性可用于绵羊育种计划的分子标记辅助选择。
