Department of Hepatobiliary Surgery, Hebei Provincial General Hospital, Shijiazhuang 050051, China.
Hepatobiliary Pancreat Dis Int. 2011 Feb;10(1):78-82. doi: 10.1016/s1499-3872(11)60011-8.
Hepatic ischemia-reperfusion injury is a common phenomenon in hepatic surgical procedures and can result in further severe damage. This study aimed to investigate the protective effects of glutamine preconditioning on hepatic ischemia-reperfusion injury in rats and its dose-dependency.
Thirty-two healthy male Wistar rats were randomly divided into four groups (n=8 per group). One group received 0.9% NaCl (control) and the other three received glutamine (Gln groups) 4 hours before ischemia. The Gln groups were named GL, GM, and GH according to the glutamine dose. The liver was subjected to 1 hour of ischemia and 2 hours of reperfusion. Two hours later, the levels of alanine aminotransferase (ALT), intracellular free calcium (Ca2+), and activity of Na+/K+ adenosine triphosphatase (ATPase) and superoxide dismutase (SOD) were assessed, and liver tissue sections were examined under a microscope.
The Gln and control groups differed in the concentration of intracellular free calcium (P<0.05), and the activity of Na+/K+ ATPase and SOD in the Gln groups was higher than in the control group (P<0.05). The ALT level was lower in the GM and GH groups than in the control group (P<0.05). The levels of Na+/K+ ATPase and SOD rose gradually with increasing glutamine dose (P<0.05), and the concentration of Ca2+ declined gradually with increasing glutamine dose (P<0.05). The degree of hepatocyte injury was milder in the Gln groups than in the control group.
Glutamine preconditioning protected effectively against hepatic ischemia-reperfusion injury. These protective effects were related to the dose of glutamine and due to the reduction of intracellular calcium overload and the improvements in the activity of Na+/K+ ATPase and SOD.
肝缺血再灌注损伤是肝外科手术中的常见现象，可导致进一步的严重损伤。本研究旨在探讨谷氨酰胺预处理对大鼠肝缺血再灌注损伤的保护作用及其剂量依赖性。
32 只健康雄性 Wistar 大鼠随机分为四组（每组 8 只）。一组给予 0.9%生理盐水（对照组），其余三组在缺血前 4 小时给予谷氨酰胺（Gln 组）。根据谷氨酰胺剂量，Gln 组分别命名为 GL、GM 和 GH。肝脏缺血 1 小时，再灌注 2 小时。2 小时后，检测丙氨酸氨基转移酶（ALT）、细胞内游离钙（Ca2+）、Na+/K+三磷酸腺苷酶（ATPase）和超氧化物歧化酶（SOD）活性，并在显微镜下观察肝组织切片。
Gln 组和对照组细胞内游离钙浓度不同（P<0.05），Gln 组 Na+/K+ATPase 和 SOD 活性高于对照组（P<0.05）。GM 和 GH 组的 ALT 水平低于对照组（P<0.05）。随着谷氨酰胺剂量的增加，Na+/K+ATPase 和 SOD 水平逐渐升高（P<0.05），Ca2+浓度逐渐降低（P<0.05）。Gln 组肝细胞损伤程度较对照组减轻。
谷氨酰胺预处理能有效保护肝缺血再灌注损伤。这些保护作用与谷氨酰胺剂量有关，可能与减轻细胞内钙超载及提高 Na+/K+ATPase 和 SOD 活性有关。
