Center for Oxygen Microscopy and Imaging (COMI), Department of Chemistry, Aarhus University, Århus C, Denmark.
Photochem Photobiol. 2011 May-Jun;87(3):671-9. doi: 10.1111/j.1751-1097.2011.00900.x. Epub 2011 Feb 22.
The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green(®) (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.
开发高效且选择性的活性氧荧光探针，特别是单线态氧的荧光探针，目前非常重要。在这项研究中，考察了商品化的单线态氧荧光探针 Singlet Oxygen Sensor Green(®)（SOSG）的光化学反应行为。尽管有文献报道相反的结果，但本文提供的数据表明，SOSG 实际上可以被整合到活的哺乳动物细胞中。然而，出于多种原因，在使用 SOSG 时必须谨慎。首先，我们证明了 SOSG 与单线态氧反应的直接产物本身就是一种有效的单线态氧光敏剂。其次，SOSG 似乎可以有效地与蛋白质结合，这反过来又会影响细胞摄取以及细胞内的行为。因此，不正确地使用 SOSG 会导致光敏单线态氧产生的产率产生误导性数据，并且还可能导致被研究体系中发生与光氧化作用相关的不良反应。
