Cui Kaixi, Li Tianzheng, Ma Yifei, Zhang Chuanjin, Zhang Ke, Qi Chao, Cai Kaiyong
Hongshen Honors School, Chongqing University, Chongqing 400044, China.
Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing 400044, China.
J Funct Biomater. 2025 Aug 21;16(8):304. doi: 10.3390/jfb16080304.
Ultrasound-responsive nanomaterials represent a promising approach for achieving non-invasive and localized drug delivery within tumor microenvironments. In this study, we developed a piezocatalysis-assisted hydrogel system that integrates reactive oxygen species (ROS) generation with stimulus-responsive drug release. The platform combines piezoelectric barium titanate (BTO) nanoparticles with a ROS-sensitive hydrogel matrix, forming an ultrasound-activated dual-function therapeutic system. Upon ultrasound irradiation, the BTO nanoparticles generate ROS-predominantly hydroxyl radicals (OH) and singlet oxygen (O)-through the piezoelectric effect, which triggers hydrogel degradation and facilitates the controlled release of encapsulated therapeutic agents. The composition and kinetics of ROS generation were evaluated using radical scavenging assays and fluorescence probe techniques, while the drug release behavior was validated under simulated oxidative environments and acoustic fields. Structural and compositional characterizations (TEM, XRD, and XPS) confirmed the quality and stability of the nanoparticles, and cytocompatibility was assessed using 3T3 fibroblasts. This synergistic strategy, combining piezocatalytic ROS generation with hydrogel disintegration, demonstrates a feasible approach for designing responsive nanoplatforms in ultrasound-mediated drug delivery systems.
超声响应性纳米材料是一种在肿瘤微环境中实现非侵入性和局部药物递送的有前景的方法。在本研究中，我们开发了一种压电催化辅助水凝胶系统，该系统将活性氧（ROS）生成与刺激响应性药物释放相结合。该平台将压电钛酸钡（BTO）纳米颗粒与ROS敏感水凝胶基质相结合，形成了一种超声激活的双功能治疗系统。在超声照射下，BTO纳米颗粒通过压电效应产生活性氧，主要是羟基自由基（OH）和单线态氧（O），这触发了水凝胶的降解并促进了封装治疗剂的控释。使用自由基清除试验和荧光探针技术评估了ROS生成的组成和动力学，同时在模拟氧化环境和声场下验证了药物释放行为。结构和组成表征（TEM、XRD和XPS）证实了纳米颗粒的质量和稳定性，并使用3T3成纤维细胞评估了细胞相容性。这种将压电催化ROS生成与水凝胶崩解相结合的协同策略，展示了一种在超声介导的药物递送系统中设计响应性纳米平台的可行方法。
