Schettler Volker, Völker Katrin, Schulz Egbert G, Wieland Eberhard
Center of Nephrology Göttingen, Göttingen, Germany.
Ther Apher Dial. 2011 Feb;15(1):105-12. doi: 10.1111/j.1744-9987.2010.00861.x. Epub 2010 Sep 26.
Lipid apheresis treatment has been suggested to cause oxidative stress. Cells respond to oxidative stress in many ways, including, among others, altered gene expressions. In the present investigation we investigated whether the gene expression of known stress genes was affected in the WBCs of patients undergoing lipid apheresis. For this purpose cellular early-growth-response gene-1 (Egr-1), c-Jun, c-Fos, and heat shock protein 70 (Hsp70) mRNA expression was followed before and immediately after lipid apheresis treatments (N=24). Gene expression was determined by quantitative reverse transcription-polymerase chain reaction. With the exception of c-Fos, the expression of Egr-1, c-Jun, and Hsp70 mRNA was not affected in WBCs by a single lipid apheresis treatment (median [16th percentile; 84 th percentile]): Egr-1, before 0.30 (0.13; 0.53), after 0.31 (0.14; 1.33); c-Jun, before 0.03 (0.03; 0.16), after 0.05 (0.03; 0.18); Hsp70, before 0.49 (0.23; 1.07), after 0.53 (0.20; 1.61)). Expression of c-Fos was significantly decreased (P<0.01) after lipid apheresis treatment (before 2.18 [1.06; 5.27], after 1.65 [0.74; 4.12]). Hsp70 and c-Fos expression in lipid apheresis patients was not different from that in 35 healthy blood donors, whereas Egr-1 and c-Jun were significantly decreased (P<0.05) in lipid apheresis patients when compared to controls (Egr-1 0.96 [0.42; 1.83], c-Jun 0.64 [0.40; 0.98], c-Fos 2.77 [1.32; 4,02], Hsp70 0.43 [0.28; 0.61]). These results show that lipid apheresis procedures do not induce stress gene expression in WBCs. Therefore, all the lipid apheresis systems used seem to be safe with respect to oxidative stress and other injuries induced in WBCs due to contact with extracorporeal tubing and membranes.
脂质分离治疗被认为会引发氧化应激。细胞对氧化应激有多种反应方式，其中包括基因表达的改变。在本研究中，我们调查了接受脂质分离治疗患者的白细胞中已知应激基因的表达是否受到影响。为此，在脂质分离治疗前及治疗后即刻（N = 24），对细胞早期生长反应基因-1（Egr-1）、c-Jun、c-Fos和热休克蛋白70（Hsp70）的mRNA表达进行了跟踪检测。基因表达通过定量逆转录-聚合酶链反应来确定。除了c-Fos外，单次脂质分离治疗对白细胞中Egr-1、c-Jun和Hsp70 mRNA的表达没有影响（中位数[第16百分位数；第84百分位数]）：Egr-1，治疗前0.30（0.13；0.53），治疗后0.31（0.14；1.33）；c-Jun，治疗前0.03（0.03；0.16），治疗后0.05（0.03；0.18）；Hsp70，治疗前0.49（0.23；1.07），治疗后0.53（0.20；1.61）。脂质分离治疗后c-Fos的表达显著降低（P<0.01）（治疗前2.18[1.06；5.27]，治疗后1.65[0.74；4.12]）。脂质分离治疗患者中Hsp70和c-Fos的表达与35名健康献血者无异，而与对照组相比，脂质分离治疗患者中Egr-1和c-Jun的表达显著降低（P<0.05）（Egr-1 0.96[0.42；1.83]，c-Jun 0.64[0.40；0.98]，c-Fos 2.77[1.32；4.02]，Hsp70 0.43[0.28；0.61]）。这些结果表明，脂质分离程序不会诱导白细胞中应激基因的表达。因此，就氧化应激以及因与体外管路和膜接触而在白细胞中引发的其他损伤而言，所使用的所有脂质分离系统似乎都是安全的。
