Yu H, Jin Z C, Yu Y N, Chen X R
Department of Pathophysiology, Zhejiang Medical University, Hangzhou.
Chin Med J (Engl). 1990 Dec;103(12):1003-7.
It was found that modified pulse induction could produce SOS responses of the sfi gene in 15 minutes. The responses peaked at 30-60 minutes and were twice as strong as those by persistent induction. The modified pulse induction could therefore more truly reflect the inducing effect. However, the SOS-inducing kinetics of MMC and 4NQO was not identical suggesting distinct kinetic patterns of induction by different mutagens. A suppressive effect on 4NQO-induced SOS response by cinnamic aldehyde and diallyl trisulfate was also observed. The effect might be due to an interference with the metabolic activation of 4NQO or breakdown of 4NQO by the bacteria.
研究发现，改良脉冲诱导可在15分钟内使sfi基因产生SOS反应。反应在30 - 60分钟达到峰值，强度是持续诱导的两倍。因此，改良脉冲诱导能更真实地反映诱导效果。然而，丝裂霉素C（MMC）和4 - 硝基喹啉 - N - 氧化物（4NQO）的SOS诱导动力学并不相同，这表明不同诱变剂的诱导动力学模式不同。还观察到肉桂醛和二烯丙基三硫化物对4NQO诱导的SOS反应有抑制作用。这种作用可能是由于干扰了4NQO的代谢活化或细菌对4NQO的分解。
