Kravchenko L V, Tutel'ian V A
Vopr Pitan. 1990 Nov-Dec(6):64-8.
Activity of microsomal UDP-glucuronosyl transferase and cytosol glutathione transferase was studied in the liver and small intestine mucosa of rats which received rations with 5.18 and 33% of protein, during 2 months. Activity of UDP-glucuronosyl transferase GT1-isoform both in native and in detergent-activated microsomes of the liver increased with protein deficiency in the ration, while GT2-isoform activity and that of three glutathione transferase forms decreased. Excess of protein in the ration led to a growth of conjugation enzyme activity in the liver. The food protein level influenced the degree of GT1 and GT2 induction by benzyl but it did not change the capacity for induction of glutathione transferase. Activity of conjugation enzymes in the small intestine mucosa of rats to a lesser degree depended on the protein level in the ration.
对喂食蛋白质含量分别为5.18%和33%的日粮达2个月的大鼠肝脏和小肠黏膜中的微粒体UDP-葡萄糖醛酸基转移酶及胞质谷胱甘肽转移酶活性进行了研究。日粮中蛋白质缺乏时，肝脏天然微粒体及经去污剂激活的微粒体中的UDP-葡萄糖醛酸基转移酶GT1同工型活性均增加，而GT2同工型活性及三种谷胱甘肽转移酶形式的活性降低。日粮中蛋白质过量导致肝脏中结合酶活性增强。食物蛋白质水平影响苄基对GT1和GT2的诱导程度，但不改变谷胱甘肽转移酶的诱导能力。大鼠小肠黏膜中结合酶的活性在较小程度上取决于日粮中的蛋白质水平。
