Vodyanoy Vitaly, Pustovyy Oleg, Globa Ludmila, Sorokulova Iryna
Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA ; School of Kinesiology, Auburn University, Auburn, AL 36849, USA ; Edward Via College of Osteopathic Medicine, Auburn, AL 36849, USA.
Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA.
Evid Based Complement Alternat Med. 2015;2015:361974. doi: 10.1155/2015/361974. Epub 2015 Aug 25.
In the 1960s Bong Han Kim discovered and characterized a new vascular system. He was able to differentiate it clearly from vascular blood and lymph systems by the use of a variety of methods, which were available to him in the mid-20th century. He gave detailed characterization of the system and created comprehensive diagrams and photographs in his publications. He demonstrated that this system is composed of nodes and vessels, and it was responsible for tissue regeneration. However, he did not disclose in detail his methods. Consequently, his results are relatively obscure from the vantage point of contemporary scientists. The stains that Kim used had been perfected and had been in use for more than 100 years. Therefore, the names of the stains were directed to the explicit protocols for the usage with the particular cells or molecules. Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method. In this present work, we have been able to disclose staining methods used by Kim.
20世纪60年代,奉韩金发现并描述了一种新的血管系统。他通过使用20世纪中叶可用的各种方法,能够将其与血管血液和淋巴系统清楚地区分开来。他对该系统进行了详细描述,并在其出版物中绘制了全面的图表和照片。他证明这个系统由节点和血管组成,并且负责组织再生。然而,他没有详细披露他的方法。因此,从当代科学家的角度来看,他的结果相对模糊。金使用的染色剂已经完善并使用了100多年。因此,染色剂的名称指向了与特定细胞或分子一起使用的明确方案。传统上,通常没有必要描述所使用的方法,除非它与原始方法有显著偏差。在本研究中,我们已经能够披露金所使用的染色方法。