Yaman Agaoglu Fulya, Kovancilar Müge, Dizdar Yavuz, Darendeliler Emin, Holdenrieder Stefan, Dalay Nejat, Gezer Ugur
Department of Radiation Oncology, Istanbul University Oncology Institute, 34390 Capa, Istanbul, Turkey.
Tumour Biol. 2011 Jun;32(3):583-8. doi: 10.1007/s13277-011-0154-9. Epub 2011 Jan 28.
In addition to their potential as tissue-based markers for cancer classification and prognostication, the study of microRNAs (miRNAs) in blood circulation is also of interest. In the present study, we investigated the amounts of three cancer-related miRNAs, miR-21, -141, and -221 in blood plasma of prostate cancer (PCa) patients. A cohort of 51 patients with PCa was enrolled into the study, and miRNAs were measured in two subgroups, with localized/local advanced or metastatic PCa. A group of 20 healthy individuals served as the control group. miRNAs were quantified from the total RNA fraction using 200 μl plasma and the small RNA molecule RNU1A as a control for normalizing the miRNA amounts in circulation. We found similar levels of three miRNAs in healthy subjects with median values of 0.039, 0.033 and 0.04, respectively; (p = n.s.). In the patients, the miRNA levels were higher, with miR-21 being the highest (median, 1.51). The miR-221 levels were intermediate (median, 0.71) while the miR-141 displayed the lowest levels (median, 0.051). The differences between the control group and the patients were highly significant for the miR-21 (p < 0.001; area under the curve (AUC), 88%) and -221 (p < 0.001; AUC, 83%) but not for the miR-141 (p = 0.2). In patients diagnosed with metastatic PCa, levels of all three miRNAs were significantly higher than in patients with localized/local advanced disease where the difference for the miR-141 was most pronounced (p< 0.001; AUC, 75.5%). In conclusion, analysis of miR-21, -141, and -221 in blood of PCa patients reveals varying patterns of these molecules in clinical subgroups of PCa.
除了作为基于组织的癌症分类和预后标志物的潜力外，对血液循环中微小RNA（miRNA）的研究也备受关注。在本研究中，我们调查了前列腺癌（PCa）患者血浆中三种与癌症相关的miRNA，即miR-21、-141和-221的含量。51例PCa患者被纳入研究，并在两个亚组中测量miRNA，分别为局限性/局部晚期或转移性PCa。20名健康个体作为对照组。使用200μl血浆和小RNA分子RNU1A从总RNA组分中对miRNA进行定量，以标准化循环中miRNA的量。我们发现健康受试者中三种miRNA的水平相似，中位数分别为0.039、0.033和0.04；（p =无显著性差异）。在患者中，miRNA水平较高，其中miR-21最高（中位数，1.51）。miR-221水平居中（中位数，0.71），而miR-141水平最低（中位数，0.051）。对照组与患者之间miR-21（p < 0.001；曲线下面积（AUC），88%）和-221（p < 0.001；AUC，83%）差异高度显著，但miR-141差异不显著（p = 0.2）。在诊断为转移性PCa的患者中，所有三种miRNA的水平均显著高于局限性/局部晚期疾病患者，其中miR-141的差异最为明显（p < 0.001；AUC，75.5%）。总之，对PCa患者血液中miR-21、-141和-221的分析揭示了这些分子在PCa临床亚组中的不同模式。
