Cellular and Molecular Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
Inflammation. 2012 Feb;35(1):138-42. doi: 10.1007/s10753-011-9298-4.
Peripheral blood fibrocytes make up a newly identified leukocyte subpopulation that displays fibroblast-like properties. These blood-borne cells can rapidly enter the site of injury at the same time as circulating inflammatory cells. Marrow stroma includes a subpopulation of undifferentiated cells that are capable of becoming one of a number of phenotypes, including chondrocytes, osteoblasts, adipocytes, and fibroblasts. Adult human bone marrow contains a minority population of bone marrow mesenchymal stem cells (BMSCs) that contribute to the regeneration of tissues such as bone, cartilage, muscle, ligaments, tendons, fat, and stroma. Evidence that these BMSCs are pluripotent, rather than being a mixture of committed progenitor cells each with a restricted potential, includes their rapid proliferation in culture. We hypothesized that peripheral blood mesenchymal stem cells (PBMSCs) and BMSCs have an effective role in wound healing. In this study, we identified and quantified the marrow stem cells (MSCs) derived from blood and bone marrow recruited and migrated to the wound site. Our results show that the synergistic effects of transforming growth factor-beta (TGF-β) and basic fibroblast growth factor (b-FGF) lead to a significant increase in migration and recruitment of both PBMSCs and BMSCs to the wound site, with more potent effects on PBMSCs as compared with BMSCs. Reverse transcription polymerase chain reaction of collagen type I (COL1A1) transcripts (348 bp) confirmed that TGF-β and b-FGF activate collagen I (production in marrow stem cells at higher transcription levels), with more vigorous effects of TGF-β on PBMSCs as compared with the same condition on BMSCs.
外周血成纤维细胞构成了一个新鉴定的白细胞亚群，其具有成纤维细胞样特性。这些血液来源的细胞可以与循环炎症细胞一起迅速进入损伤部位。骨髓基质包括一群未分化的细胞，这些细胞能够成为多种表型之一，包括软骨细胞、成骨细胞、脂肪细胞和成纤维细胞。成人骨髓中含有少数骨髓间充质干细胞（BMSCs），它们有助于骨骼、软骨、肌肉、韧带、肌腱、脂肪和基质等组织的再生。这些 BMSCs 具有多能性，而不是由具有有限潜能的定向祖细胞混合物组成的证据包括它们在培养中快速增殖。我们假设外周血间充质干细胞（PBMSCs）和 BMSCs 在伤口愈合中有有效作用。在这项研究中，我们鉴定和量化了源自血液和骨髓的招募和迁移到伤口部位的骨髓干细胞（MSCs）。我们的结果表明，转化生长因子-β（TGF-β）和碱性成纤维细胞生长因子（b-FGF）的协同作用导致 PBMSCs 和 BMSCs 向伤口部位的迁移和募集显著增加，与 BMSCs 相比，对 PBMSCs 的作用更强。I 型胶原蛋白（COL1A1）转录物（348 bp）的逆转录聚合酶链反应证实 TGF-β 和 b-FGF 激活胶原蛋白 I（在骨髓干细胞中以更高的转录水平产生），与 BMSCs 相同条件相比，TGF-β 对 PBMSCs 的作用更强。
