Singer S W, Reddy A P, Gladden J M, Guo H, Hazen T C, Simmons B A, VanderGheynst J S
Joint BioEnergy Institute, Emeryville, CA, USA Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA Department of Biological and Agricultural Engineering, University of California, Davis, CA, USA Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA Biomass Science and Conversion Technology Department, Sandia National Laboratories, Livermore, CA, USA.
J Appl Microbiol. 2011 Apr;110(4):1023-31. doi: 10.1111/j.1365-2672.2011.04959.x. Epub 2011 Feb 21.
This work aimed to characterize microbial tolerance to 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid that has emerged as a novel biomass pretreatment for lignocellulosic biomass.
Enrichment experiments performed using inocula treated with [C2mim][OAc] under solid and liquid cultivation yielded fungal populations dominated by Aspergilli. Ionic liquid-tolerant Aspergillus isolates from these enrichments were capable of growing in a radial plate growth assay in the presence of 10% [C2mim][OAc]. When a [C2mim][OAc]-tolerant Aspergillus fumigatus strain was grown in the presence of switchgrass, endoglucanases and xylanases were secreted that retained residual enzymatic activity in the presence of 20% [C2mim][OAc].
The results of the study suggest that tolerance to ionic liquids is a general property of the Aspergilli.
Tolerance to an industrially important ionic liquid was discovered in a fungal genera that is widely used in biotechnology, including biomass deconstruction.
本研究旨在表征微生物对1-乙基-3-甲基咪唑醋酸盐（[C2mim][OAc]）的耐受性，该离子液体已成为一种用于木质纤维素生物质预处理的新型生物质预处理剂。
在固体和液体培养条件下，使用经[C2mim][OAc]处理的接种物进行富集实验，得到以曲霉菌为主的真菌群体。从这些富集物中分离出的耐离子液体曲霉菌株能够在含有10%[C2mim][OAc]的平板生长试验中生长。当耐[C2mim][OAc]的烟曲霉菌株在柳枝稷存在的情况下生长时，会分泌内切葡聚糖酶和木聚糖酶，这些酶在含有20%[C2mim][OAc]的情况下仍保留残余酶活性。
研究结果表明，对离子液体的耐受性是曲霉菌的一个普遍特性。
在广泛应用于生物技术（包括生物质解构）的真菌属中发现了对一种具有工业重要性的离子液体的耐受性。
