Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian 223300, China.
Bioresour Technol. 2015 Apr;181:18-25. doi: 10.1016/j.biortech.2014.12.080. Epub 2014 Dec 31.
In this study, microbial communities from chemicals polluted microhabitats were cultured with the addition of imidazolium-based ionic liquid (IL) to enrich for IL-tolerant microbes. A strain of Fusarium oxysporum BN producing cellulase from these enrichments was capable of growing in 10% (w/v) 1-ethyl-3-methylimidazolium phosphinate, much higher than the normal IL concentrations in the lignocellulose regenerated from ILs. Cellulase secreted by the strain showed high resistance to ILs based on phosphate and sulfate radicals, evidencing of a high conformational stability in relevant media. Gratifyingly, F. oxysporum BN can directly convert IL-pretreated rice straw to bioethanol via consolidated bioprocessing (I-CBP). At optimum fermentation condition, a maximum ethanol yield of 0.125 g ethanol g(-1) of rice straw was finally obtained, corresponding to 64.2% of the theoretical yield.
在这项研究中，向受化学污染物污染的小生境中的微生物群落中添加基于咪唑的离子液体 (IL)，以富集耐受 IL 的微生物。从这些富集物中产生纤维素酶的尖孢镰刀菌 BN 菌株能够在 10%（w/v）的 1-乙基-3-甲基咪唑磷酸盐水溶液中生长，远高于从 IL 中再生的木质纤维素中的正常 IL 浓度。该菌株分泌的纤维素酶对基于磷酸盐和硫酸盐自由基的 IL 表现出高抗性，证明其在相关介质中具有高构象稳定性。令人高兴的是，尖孢镰刀菌 BN 可以通过整合生物加工 (I-CBP) 直接将 IL 预处理的水稻秸秆转化为生物乙醇。在最佳发酵条件下，最终获得了 0.125g 乙醇 g(-1)稻草的最大乙醇产率，相当于理论产率的 64.2%。
