Laboratório de Farmacologia da Inflamação e do Óxido Nítrico, ICB, Universidade Federal do Rio de Janeiro, Brazil.
J Ethnopharmacol. 2011 Apr 12;134(3):725-32. doi: 10.1016/j.jep.2011.01.027. Epub 2011 Jan 26.
Hyptis pectinata Poit (Lamiaceae) is grown in the northeastern regions of Brazil and is popularly known as "sambacaitá" or "canudinho". It is extensively used in folk medicine to treat inflammatory conditions, bacterial infections, pain, and cancer.
Hyptis pectinata essential oil (EO, 10, 30, and 100mg/kg, p.o.) and the reference drugs morphine (5mg/kg, p.o.) and acetylsalicylic acid (ASA, 200mg/kg, p.o.) were evaluated using models for analgesia (acetic acid-induced contortions and hot plate) or inflammation (formalin-induced licking response and the subcutaneous air-pouch model). To elucidate the EO's mechanism of action, animals were pre-treated with the opioid receptor antagonist naloxone (1mg/kg, i.p.), the cholinergic antagonist atropine (1mg/kg, i.p.), or l-nitro arginine methyl ester (l-NAME, 3mg/kg, i.p.) 30 min prior to the oral administration of the EO.
The EO significantly inhibited the number of writhings and the time the animals spent licking their formalin-injected paws (second phase). The EO, at doses of 30 and 100mg/kg, increased baseline measurements and area under the curve measurements in the hot plate model, respectively. The administration of naloxone reversed the antinociceptive effect of the EO in the hot plate model. l-NAME significantly reversed the effects of the EO in the contortions and hot plate models. Atropine completely reversed the antinociceptive activity of the EO in all models. Additionally, the EO inhibited the inflammatory process induced by subcutaneous carrageenan injection by reducing cell migration, exudate volume, protein concentration, and inflammatory mediators (nitric oxide, prostaglandin E2, IL-6, and TNF-α) produced in the pouch.
Our results indicate that the Hyptis pectinata essential oil exhibits antinociceptive effects, likely mediated by opioid and cholinergic receptors, and anti-inflammatory activity through the inhibition of nitric oxide and PGE2 production.
巴西东北部广泛种植的唇形科植物 Hypis pectinata Poit，俗称“sambacaitá”或“canudinho”。在民间医学中，它被广泛用于治疗炎症、细菌感染、疼痛和癌症。
采用醋酸扭体和热板模型评估 Hypis pectinata 精油（10、30 和 100mg/kg，po）和参考药物吗啡（5mg/kg，po）和乙酰水杨酸（200mg/kg，po）的镇痛作用；采用福尔马林诱导舔舐反应和皮下气囊模型评估其抗炎作用。为阐明精油的作用机制，动物预先用阿片受体拮抗剂纳洛酮（1mg/kg，ip）、胆碱能拮抗剂阿托品（1mg/kg，ip）或 l-硝基精氨酸甲酯（l-NAME，3mg/kg，ip）预处理，30min 后给予精油口服。
精油显著抑制扭体次数和动物舔舐福尔马林注射后爪子的时间（第二阶段）。在热板模型中，精油在 30 和 100mg/kg 剂量下分别增加了基础测量值和曲线下面积测量值。纳洛酮逆转了热板模型中精油的镇痛作用。l-NAME 显著逆转了扭体和热板模型中精油的作用。阿托品完全逆转了精油在所有模型中的镇痛活性。此外，精油通过减少细胞迁移、渗出物体积、蛋白浓度和炎症介质（一氧化氮、前列腺素 E2、IL-6 和 TNF-α）的产生，抑制了皮下角叉菜胶注射引起的炎症过程。
我们的结果表明，Hypis pectinata 精油具有镇痛作用，可能通过阿片受体和胆碱能受体介导，通过抑制一氧化氮和 PGE2 的产生发挥抗炎作用。
