State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, China.
Bioengineered. 2021 Dec;12(2):9860-9871. doi: 10.1080/21655979.2021.1996149.
chvar. essential oil (BEO, 18.2% v/v borneol) is a by-product of steam distillation to produce natural crystalline borneol (NCB, 98.4% v/v borneol). Given the known medicinal properties of borneol, the analgesic function and safety were studied. Horn's method and the Draize test revealed a gender difference in mice regarding acute oral LD, i.e., low-toxicity to female mice (2749 mg/kg), but practically nontoxic to male mice (5081 mg/kg). There was no acute and skin or eye irritation when BEO was applied directly, if the BEO concentration was less than 50%. The analgesic effect of BEO was evaluated by the glacial acetic acid-induced writhing pain model. Continuous topical application of BEO to the abdomen of mice for 6 d, significantly reduced observed writhing in mice ( < 0.001) with a strong dose-response relationship (r = -0.9006). Concomitantly, the levels of the serum pain-related mediators, prostaglandin E (PGE) and transient receptor potential melastatin-8 (TRPM8) were significantly reduced ( < 0.001), and the latter showed a strong dose-response relationship (r = -0.9427). Therefore, BEO had similar analgesic functions to borneol and was demonstrated to be safe for medicinal use.
苍术酮（BEO，18.2%v/v 龙脑）是水蒸气蒸馏生产天然结晶龙脑（NCB，98.4%v/v 龙脑）的副产品。鉴于龙脑的已知药用特性，研究了其镇痛功能和安全性。Horn 法和 Draize 试验揭示了雌雄小鼠在急性口服 LD 方面存在性别差异，即雌鼠的低毒性（2749mg/kg），而雄鼠实际上无毒（5081mg/kg）。如果 BEO 浓度低于 50%，直接应用 BEO 不会引起急性皮肤或眼睛刺激。通过醋酸致小鼠扭体疼痛模型评价 BEO 的镇痛作用。连续 6d 局部应用 BEO 于小鼠腹部，明显减少观察到的小鼠扭体次数（<0.001），呈强剂量-反应关系（r=-0.9006）。同时，血清疼痛相关介质前列腺素 E（PGE）和瞬时受体电位 melastatin-8（TRPM8）的水平显著降低（<0.001），后者呈强剂量-反应关系（r=-0.9427）。因此，BEO 具有与龙脑相似的镇痛作用，且被证明可安全用于药用。
