First Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1, Towada, Aomori 034-8628, Japan.
Parasitol Res. 2011 Jul;109(1):253-6. doi: 10.1007/s00436-011-2258-y. Epub 2011 Jan 29.
The present study is the first report that describes the national survey of intestinal parasites in private household dogs brought to veterinary clinics in Japan. A total of 2,365 fresh feces were collected. Giardia-specific coproantigen was examined by ELISA kit (SNAP(®) Giardia, IDEXX Laboratories, Inc.; Maine, USA). Other intestinal parasites were determined microscopically using the formalin-ethyl acetate sedimentation technique. According to age categories, Giardia duodenalis, Cystoisospora spp., Toxocara canis, Toxascaris leonina, and Strongyloides spp., at ≦6-months-old showed significantly (P < 0.0001, P < 0.001 or P < 0.01, respectively) higher prevalence compared to >6 months old (31.5% vs. 2.3%, 9.1% vs. 0.05%, 1.8% vs. 0.4%, 1.1% vs. 0%, and 1.1% vs. 0.05%, respectively). In clinical categories, prevalences of G. duodenalis (14.8%) and Cystoisospora spp. (4.7%) in symptomatic dogs were significantly (P < 0.05, respectively) higher than those in asymptomatic ones (7.9% and 1.6%, respectively). G. duodenalis and Cystoisospora spp. were dominant parasites in private household dogs in Japan, especially ≦6-month-old dogs.
本研究首次报道了在日本兽医诊所中对私人家庭犬进行的全国性肠道寄生虫调查。共采集了 2365 份新鲜粪便。采用 ELISA 试剂盒（SNAP(®) Giardia，IDEXX Laboratories，Inc.；Maine，USA）检测贾第鞭毛虫特异性粪抗原。使用福尔马林-乙酸乙酯沉淀技术通过显微镜检查其他肠道寄生虫。根据年龄类别，6 月龄以下的犬贾第虫、隐孢子虫、犬蛔虫、狮弓蛔虫和类圆线虫的流行率明显（P < 0.0001，P < 0.001 或 P < 0.01，分别）高于 6 月龄以上（31.5%比 2.3%，9.1%比 0.05%，1.8%比 0.4%，1.1%比 0%和 1.1%比 0.05%，分别）。在临床类别中，有症状犬的犬贾第虫（14.8%）和隐孢子虫（4.7%）的流行率明显（P < 0.05，分别）高于无症状犬（7.9%和 1.6%，分别）。犬贾第虫和隐孢子虫是日本私人家庭犬中的主要寄生虫，尤其是 6 月龄以下的犬。
