一种基于序列的新型 coa 基因分型方法，用于区分医院内耐甲氧西林金黄色葡萄球菌分离株。
A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates.
机构信息
Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
出版信息
Ir J Med Sci. 2011 Jun;180(2):463-8. doi: 10.1007/s11845-011-0680-x. Epub 2011 Jan 29.
AIM
To evaluate a novel sequence-based coa typing method in classification for nosocomial methicillin-resistant Staphylococcus aureus.
METHOD
D1 and D2 regions of coa gene were used to discriminate methicillin-resistant Staphylococcus aureus and compared with other classical sequence-based methods with discriminate power.
RESULTS
All MRSA isolates were divided into 11 types and about 20 subtypes with coa typing method here. The discriminate power of this coa typing method was better than MLST or spa method.
CONCLUSION
It was demonstrated that this coa method is a simple, rapid, and effective technique applicable for outbreak or local nosocomial MRSA investigations in the future.
目的
评估一种新的基于序列的 coa 分型方法在医院耐甲氧西林金黄色葡萄球菌分类中的应用。
方法
使用 coa 基因的 D1 和 D2 区来区分耐甲氧西林金黄色葡萄球菌，并与其他具有鉴别能力的经典基于序列的方法进行比较。
结果
所有耐甲氧西林金黄色葡萄球菌分离株均采用 coa 分型方法分为 11 种和 20 种左右的亚型。该 coa 分型方法的鉴别能力优于 MLST 或 spa 方法。
结论
该 coa 方法简单、快速、有效，适用于未来医院耐甲氧西林金黄色葡萄球菌暴发或局部感染的调查。
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