伊朗西南部临床分离株中四环素抗性基因、氨基糖苷类修饰酶及凝固酶基因分型的检测
Detection of tetracycline resistance genes, aminoglycoside modifying enzymes, and coagulase gene typing of clinical isolates of in the Southwest of Iran.
作者信息
Khoramrooz Seyed Sajjad, Dolatabad Samin Alipoor, Dolatabad Fatemeh Mostafapour, Marashifard Masoud, Mirzaii Mehdi, Dabiri Hossein, Haddadi Azam, Rabani Seyed Mohammadreza, Shirazi Hamid Reza Ghaffarian, Darban-Sarokhalil Davood
机构信息
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Department of Biology, Faculty of Science, Karaj Branch, Islamic Azad University, Karaj, Iran.
出版信息
Iran J Basic Med Sci. 2017 Aug;20(8):912-919. doi: 10.22038/IJBMS.2017.9114.
OBJECTIVES
The aim of the present study was to determine the aminoglycoside modifying enzymes (AMEs) encoded genes, tetracycline resistance genes, and the based typing of isolates in the Southwest of Iran.
MATERIALS AND METHODS
Antimicrobial susceptibility of isolates was carried out by agar disk diffusion methods. Two sets of multiplex PCR mixture were used for detection of AME genes and genes. All of the isolates were typed with the coagulase gene typing method. Of the 121 isolates, 29.75% and 47.93% were resistant to at least one aminoglycosides and tetracyclines, respectively.
RESULTS
The was the most frequent gene (97.22%), and and genes were detected in 61.11% and 11.11% of aminoglycoside resistant isolates, respectively. The K and M genes were detected in 82.75% and 56.9% of tetracycline resistant isolates, respectively. Overall 31.4% of isolates were MRSA. Totally 17 distinct gene RFLP patterns, numbered C1 to C17, were observed. The C5 was the most frequent type with 31 isolates.
CONCLUSION
The and genes were the most important genes contributing to aminoglycosides resistance, while resistance to tetracyclines was mediated by K and M genes. Interestingly all with C5 as the most prevalent -type were resistant to at least one of the aminoglycoside antibiotics and tetracycline simultaneously. Moreover, 30 out of 31 isolates with this type were MRSA, indicating the importance of the C5 -type in MRSA strains and also in isolates that were resistant to aminoglycosides and tetracycline.
目的
本研究旨在确定伊朗西南部分离株中编码氨基糖苷修饰酶（AMEs）的基因、四环素抗性基因以及基于凝固酶基因分型的菌株类型。
材料与方法
采用琼脂纸片扩散法检测分离株的抗菌药敏性。使用两组多重PCR混合物检测AME基因和四环素抗性基因。所有分离株均采用凝固酶基因分型方法进行分型。在121株分离株中，分别有29.75%和47.93%对至少一种氨基糖苷类药物和四环素耐药。
结果
aac（6′）-Ib-cr是最常见的基因（97.22%），在61.11%和11.11%的氨基糖苷类耐药分离株中分别检测到ant（3″）-Ia和aph（3′）-IIIa基因。K和M基因分别在82.75%和56.9%的四环素耐药分离株中检测到。总体而言，31.4%的分离株为耐甲氧西林金黄色葡萄球菌（MRSA）。共观察到17种不同的凝固酶基因限制性片段长度多态性（RFLP）模式，编号为C1至C17。C5是最常见的凝固酶基因类型，有31株。
结论
aac（6′）-Ib-cr和ant（3″）-Ia基因是导致氨基糖苷类耐药的最重要基因，而对四环素的耐药由K和M基因介导。有趣的是，所有以C5为最普遍凝固酶基因类型的菌株同时对至少一种氨基糖苷类抗生素和四环素耐药。此外，31株该凝固酶基因类型的分离株中有30株为MRSA，表明C5凝固酶基因类型在MRSA菌株以及对氨基糖苷类和四环素耐药的分离株中的重要性。
Zotero 插件