Ohtsubo K, Izumiyama N, Kuzuhara S, Mori H, Shimada H
Department of Clinical Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Acta Neuropathol. 1990;81(2):111-5. doi: 10.1007/BF00334498.
The ultrastructure of the curly fibers was examined by the transmission and immunoelectron microscopy as well as by the rapid-freeze, deep-etch and replica method. The curly fibers consisted mainly of paired helical filaments (PHF) in the neuropils, both pre- and post-synaptic. On the deep-etch replicas, PHF in the neurites had similar dimensions to those of Alzheimer's neurofibrillary tangles in the nerve cell somata, having the width of 28 to 36 nm. The two component filaments, 14 to 18 nm in width, were twisted left-handedly with about 70- to 90-nm intervals. There were also cross-bridging fibrils of about 6 nm in diameter connecting the neighboring PHF.
通过透射电子显微镜、免疫电子显微镜以及快速冷冻、深度蚀刻和复型方法对卷曲纤维的超微结构进行了检查。卷曲纤维主要由神经毡中突触前和突触后的双螺旋丝（PHF）组成。在深度蚀刻复型上，神经突中的PHF尺寸与神经细胞胞体中阿尔茨海默病神经原纤维缠结的尺寸相似，宽度为28至36纳米。两条宽度为14至18纳米的组成丝以左手螺旋方式扭曲，间隔约70至90纳米。还有直径约6纳米的交叉桥连纤维连接相邻的PHF。
